首页> 美国卫生研究院文献>Animals : an Open Access Journal from MDPI >A Simple and Efficient Semen Cryopreservation Method to Increase the Genetic Variability of Endangered Mediterranean Brown Trout Inhabiting Molise Rivers
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A Simple and Efficient Semen Cryopreservation Method to Increase the Genetic Variability of Endangered Mediterranean Brown Trout Inhabiting Molise Rivers

机译:一种简单高效的冷冻冷冻精液的方法可提高濒临莫利塞河的地中海褐鳟的遗传变异性

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摘要

The sperm cryobank is an effective strategy for protecting the biodiversity of the local brown trout population. Thus, the aim of the present work was to test, for the first time, the effectiveness of a semen cryopreservation protocol developed for cultivated salmonid fish on wild trout inhabiting the Molise rivers. Moreover, the effects of two different thawing rates (40°C/5 s and 10°C/30 s) were evaluated in vitro and in vivo. In addition, different sperm-to-egg ratios (6 × 10 :1, 4.5 ×10 :1 and 3 × 10 :1) were also investigated in vivo in order to find an effective freezing protocol useful for the creation of the first European semen cryobank for the Mediterranean brown trout ( ). From results obtained in vitro 40 °C turned out to be the best thawing rate, which in combination with a sperm-to-egg ratio of 4.5 × 10 :1 was, in vivo, the highest fertilization rate. The important results obtained in the present work corroborated the remarkable validity and effectiveness of the freezing protocol used in this study on the native trout populations from Molise.
机译:精子冷冻库是保护当地褐鳟种群生物多样性的有效策略。因此,本工作的目的是首次测试针对居住在莫利塞河上的野生鳟鱼上养殖的鲑鱼开发的精液冷冻保存方案的有效性。此外,在体外和体内评估了两种不同的解冻速率(40°C / 5 s和10°C / 30 s)的影响。此外,还对体内不同的精子/卵子比率(6×10:1、4.5×10:1和3×10:1)进行了研究,以发现有效的冷冻方法,可用于创建第一个欧洲人。用于地中海褐鳟的精液冷冻库()。从体外获得的结果可知,最佳的解冻速率是40°C,而在体内,结合4.5×10 1:1的精子/卵子比率,则是最高的受精率。在当前工作中获得的重要结果证实了本研究中用于莫利塞天然鳟鱼种群的冷冻方案的显着有效性和有效性。

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