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首页> 美国卫生研究院文献>Antioxidants >Chemopreventive Effect of β-Cryptoxanthin on Human Cervical Carcinoma (HeLa) Cells Is Modulated through Oxidative Stress-Induced Apoptosis
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Chemopreventive Effect of β-Cryptoxanthin on Human Cervical Carcinoma (HeLa) Cells Is Modulated through Oxidative Stress-Induced Apoptosis

机译：β-隐黄质对人宫颈癌（HeLa）细胞的化学预防作用可通过氧化应激诱导的细胞凋亡来调节。

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The present study was aimed to assess cellular and molecular events involved in the chemopreventive activities of β-cryptoxanthin derived from mandarin oranges ( Marc.) on human cervical carcinoma (HeLa) cells. In vitro experiments established that β-cryptoxanthin significantly inhibited the proliferation of HeLa cells with the IC value of 4.5 and 3.7 µM after 24 and 48 h of treatments, respectively. β-cryptoxanthin-treated HeLa cells exhibited enhanced levels of oxidative stress correlated with significant downregulation of anti-apoptotic Bcl-2, and upregulation of pro-apoptotic Bax mRNA expression. Moreover, β-cryptoxanthin triggered nuclear condensation and disruption of the integrity of the mitochondrial membrane, upregulated caspase-3, -7, and -9 mRNA, and enhanced activation of caspase-3 proteins, resulting in nuclei DNA damage and apoptosis of HeLa cells. Remarkably, TUNEL assay carried out to detect nuclei DNA damage showed 52% TUNEL-positive cells after treatment with a physiological concentration of β-cryptoxanthin (1.0 μM), which validates its potential as an anticancer drug of natural origin.
机译：本研究旨在评估与橘子（Marc。）衍生的β-隐黄质对人宫颈癌（HeLa）细胞化学预防活性有关的细胞和分子事件。体外实验表明，处理24小时和48小时后，β-隐黄质可以显着抑制HeLa细胞的增殖，IC值分别为4.5和3.7 µM。 β-隐黄嘌呤处理的HeLa细胞显示出较高的氧化应激水平，与抗凋亡Bcl-2的显着下调和促凋亡Bax mRNA表达的上调相关。此外，β-隐黄质触发核浓缩并破坏线粒体膜的完整性，上调caspase-3，-7和-9 mRNA的表达，并增强caspase-3蛋白的激活，导致细胞核DNA损伤和HeLa细胞凋亡。 。值得注意的是，进行TUNEL检测以检测细胞核DNA损伤后，生理浓度的β-隐黄质（1.0μM）处理后，TUNEL阳性细胞占52％，这证明了其作为天然抗癌药物的潜力。

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