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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Multicenter Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with the National Committee for Clinical Laboratory Standards M27-A Reference Method for Testing Clinical Isolates of Common and Emerging Candida spp. Cryptococcus spp. and Other Yeasts and Yeast-Like Organisms
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Multicenter Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with the National Committee for Clinical Laboratory Standards M27-A Reference Method for Testing Clinical Isolates of Common and Emerging Candida spp. Cryptococcus spp. and Other Yeasts and Yeast-Like Organisms

机译：敏感性酵母YeastOne比色抗真菌检测小组与国家临床实验室标准委员会M27-A检验常见和新兴念珠菌隐球菌和其他酵母菌和类似酵母菌的临床分离株的参考方法的多中心比较

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National Committee for Clinical Laboratory Standards (NCCLS) standard guidelines are available for the antifungal susceptibility testing of common Candida spp. and Cryptococcus neoformans, but NCCLS methods may not be the most efficient and convenient procedures for use in the clinical laboratory. MICs of amphotericin B, fluconazole, flucytosine, itraconazole, and ketoconazole were determined by the commercially prepared Sensititre YeastOne Colorimetric Antifungal Panel and by the NCCLS M27-A broth microdilution method for 1,176 clinical isolates of yeasts and yeast-like organisms, including Blastoschizomyces capitatus, Cryptococcus spp., 14 common and emerging species of Candida, Hansenula anomala, Rhodotorula spp., Saccharomyces cerevisiae, Sporobolomyces salmonicolor, and Trichosporon beigelii. Colorimetric MICs of amphotericin B corresponded to the first blue well (no growth), and MICs of the other agents corresponded to the first purple or blue well. Three comparisons of MIC pairs by the two methods were evaluated to obtain percentages of agreement: 24- and 48-h MICs and 24-h colorimetric versus 48-h reference MICs. The best performance of the YeastOne panel was with 24-h MICs (92 to 100%) with the azoles and flucytosine for all the species tested, with the exception of C. albicans (87 to 90%). For amphotericin B, the best agreement between the methods was with 48-h MIC pairs (92 to 99%) for most of the species tested. The exception was for isolates of C. neoformans (76%). These data suggest the potential value of the YeastOne panel for use in the clinical laboratory.
机译：全国临床实验室标准委员会（NCCLS）标准指南可用于常见假丝酵母菌的抗真菌药敏试验。和新型隐球菌，但NCCLS方法可能不是临床实验室中最有效，最方便的方法。两性霉素B，氟康唑，氟胞嘧啶，伊曲康唑和酮康唑的MIC分别通过商业化制备的Sensititre YeastOne比色抗真菌小组和NCCLS M27-A肉汤微稀释法测定了1,176份临床分离的酵母和类酵母菌生物体，其中包括芽孢杆菌。隐球菌属，14种常见和新兴种的念珠菌，汉逊酵母，红假单胞菌，酿酒酵母，鲑形孢菌和米色毛孢菌。两性霉素B的比色MIC对应于第一个蓝色孔（无生长），其他试剂的MIC对应于第一个紫色或蓝色孔。评估了通过两种方法对MIC对进行的三个比较，以得出一致性百分比：24小时和48小时MIC和24小时比色与48小时参考MIC。 YeastOne小组的最佳性能是使用24小时MIC（92％至100％），以及所有白色菌种（除白色念珠菌（87％至90％）外，均含唑和氟胞嘧啶）。对于两性霉素B，两种方法之间的最佳一致性是大多数被测物种均使用48小时MIC对（92％至99％）。唯一的例外是新孢梭菌的分离株（76％）。这些数据表明YeastOne面板在临床实验室中使用的潜在价值。

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