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Non-destructive label free identification of cell cycle phase in cancer cells by multispectral microscopy of autofluorescence

机译：通过自发荧光多光谱显微镜对癌细胞的细胞周期进行无损无标记鉴定

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摘要

Confocal laser scanning images of phases of the cell cycle. HeLa nuclei, blue is DAPI green is PCNA. G1 phase is distinguished by solid distribution of PCNA through the nucleus, S phase is distinguished by PCNA speckling through the nucleus and a nuclear border depending on position within S-phase (mid-S displayed here) as well as increased total DNA intensity compared to G1, G2 is distinguished by solid distribution of PCNA and twice the total DNA intensity of G1, M phase is distinguished by the exclusion of PCNA from the nucleus into the cytoplasm

机译：共聚焦激光扫描细胞周期各阶段的图像。 HeLa核，蓝色是DAPI，绿色是PCNA。 G1相的特征是PCNA穿过核的固体分布，S相的特征是PCNA穿过核和核边界散斑，这取决于S相中的位置（此处显示的是中S），以及与G1，G2的特征在于PCNA的固相分布，而G1，M期的总DNA强度则是PCNA从细胞核被排除到细胞质中的两倍

著录项

期刊名称 BMC Cancer
作者
Jared M. Campbell; Abbas Habibalahi; Saabah Mahbub; Martin Gosnell; Ayad G. Anwer; Sharon Paton; Stan Gronthos; Ewa Goldys;
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作者单位

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年(卷),期 2019(19),-1
年度 2019
页码 -1
总页数 11
原文格式 PDF
正文语种
中图分类肿瘤学;
关键词
Cell phase; Cell cycle; Multispectral; Hyperspectral; Neoplasia; Cancer;

机译：细胞期;细胞周期;多光谱;高光谱;肿瘤形成;癌症;

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专利

1. Non-destructive, label free identification of cell cycle phase in cancer cells by multispectral microscopy of autofluorescence [J] . Jared M. Campbell, Abbas Habibalahi, Saabah Mahbub, BMC Cancer . 2019,第1期

机译：非破坏性的，通过多光谱显微镜的自发荧光显微镜测定癌细胞中细胞周期阶段的自由鉴定
2. Label-free characterization of living human induced pluripotent stem cells by subcellular topographic imaging technique using full-field quantitative phase microscopy coupled with interference reflection microscopy [J] . Norikazu Sugiyama, Yasuyuki Asai, Toyohiko Yamauchi, Biomedical Optics Express . 2012,第9期

机译：使用全场定量相显微镜和干扰反射显微镜通过亚细胞地形成像技术对人类诱导的多能干细胞进行无标记表征
3. Label-free diagnosis of human hepatocellular carcinoma by multiphoton autofluorescence microscopy [J] . Tzu-Lin Sun, Yuan Liu, Ming-Chin Sung, Applied Physicsletters . 2009,第19期

机译：多光子自发荧光显微镜无标记诊断人肝细胞癌
4. Label-Free Quantitative Classification of Cancer Cells Measured by Interferometric Phase Microscopy [C] . Natan T. Shaked Conference on Lasers and Electro-Optics . 2019

机译：干涉相显微镜测量癌细胞的无标记定量分类
5. Label-free Detection of Cancer Cells with Polysilicon Sensor Chips and Biomolecule-assisted Synthesis of Shape-controlled Nanoparticles [D] . Shi, Menglu. 2015

机译：多晶硅传感器芯片和形状控制的纳米粒子的生物分子辅助合成的无标记癌细胞检测。
6. Label-free characterization of living human induced pluripotent stem cells by subcellular topographic imaging technique using full-field quantitative phase microscopy coupled with interference reflection microscopy [O] . Norikazu Sugiyama, Yasuyuki Asai, Toyohiko Yamauchi, 2012

机译：使用全场定量相显微镜和干扰反射显微镜通过亚细胞地形成像技术对人类诱导的多能干细胞进行无标记表征
7. Non-destructive, label free identification of cell cycle phase in cancer cells by multispectral microscopy of autofluorescence [O] . Jared M. Campbell, Abbas Habibalahi, Saabah Mahbub, 2019

机译：非破坏性的，通过多光谱显微镜的自发荧光显微镜测定癌细胞中细胞周期阶段的自由鉴定

Non-destructive label free identification of cell cycle phase in cancer cells by multispectral microscopy of autofluorescence

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