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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Evaluation of Automated BACTEC MGIT 960 System for Testing Susceptibility of Mycobacterium tuberculosis to Four Major Antituberculous Drugs: Comparison with the Radiometric BACTEC 460TB Method and the Agar Plate Method of Proportion
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Evaluation of Automated BACTEC MGIT 960 System for Testing Susceptibility of Mycobacterium tuberculosis to Four Major Antituberculous Drugs: Comparison with the Radiometric BACTEC 460TB Method and the Agar Plate Method of Proportion

机译：测试结核分枝杆菌对四种主要抗结核药物敏感性的自动化BACTEC MGIT 960系统的评估：与放射BACTEC 460TB方法和琼脂平板法的比例比较

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We evaluated the performance of BACTEC MGIT 960 for automated testing of the susceptibility of 133 strains of Mycobacterium tuberculosis to streptomycin, isoniazid, rifampin, and ethambutol. The BACTEC MGIT 960 results were compared with those obtained with the radiometric BACTEC 460TB system, and when there was disagreement, the method of proportion on agar plates was used as a reference method. Strains resistant to the critical concentration of streptomycin, isoniazid, or ethambutol were also tested with a second, higher concentration. The overall agreement between the two systems was 96.7%, and the 18 discrepancies were resolved in favor of BACTEC 460TB in 11 cases and in favor of BACTEC MGIT 960 in 7, a difference which was not statistically significant. Apart from the assay's low specificity for ethambutol, which was low for the radiometric assay as well, good sensitivity and specificity values characterized BACTEC MGIT 960. The average time required for completion of the test was 2.5 days shorter with BACTEC 460TB. In conclusion, BACTEC MGIT 960 appears to be a suitable replacement for the radiometric method of antimicrobial susceptibility testing of M. tuberculosis. The problem of frequent contamination of BACTEC MGIT 960 tests needs to be quickly resolved; in fact, 14 strains had to be reprocessed because of contamination.
机译：我们评估了BACTEC MGIT 960在133种结核分枝杆菌对链霉素，异烟肼，利福平和乙胺丁醇的敏感性自动测试中的性能。将BACTEC MGIT 960结果与通过辐射BACTEC 460TB系统获得的结果进行比较，如果存在分歧，则以琼脂平板上的比例法作为参考方法。还以较高的第二浓度测试了对临界浓度的链霉素，异烟肼或乙胺丁醇具有抗性的菌株。两个系统之间的总体一致性为96.7％，解决了18个差异，其中11例赞成BACTEC 460TB，而7例赞成BACTEC MGIT 960，差异无统计学意义。除了该方法对乙胺丁醇的特异性较低（对于放射分析法也同样如此）之外，BACTEC MGIT 960具有良好的灵敏度和特异性值。使用BACTEC 460TB，完成测试所需的平均时间缩短了2.5天。总之，BACTEC MGIT 960似乎是结核分枝杆菌抗菌敏感性测试的放射线测定方法的合适替代品。 BACTEC MGIT 960测试频繁污染的问题需要迅速解决。实际上，由于污染，必须对14个菌株进行后处理。

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