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FGF-induced LHX9 regulates the progression and metastasis of osteosarcoma via FRS2/TGF-β/β-catenin pathway

机译:FGF诱导的LHX9通过FRS2 /TGF-β/β-catenin途径调节骨肉瘤的进展和转移

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摘要

The expression of was up-regulated in OS tissues and cell lines. The relative expression of in normal tissues (solid dots) and OS tumor tissues (solid squares) excised from patients was assessed by RT-qPCR. mRNA levels were normalized to mRNA (n = 3). The correlation analysis between the expression of and the survival of patients with OS. Blue line: low and medium expression; red line: high expression. The relative expression of in OS cell lines 143B, U2OS, MNNG-HOS and Saos-2 was measured by RT-qPCR. hFOB.19 was the control cell line. The mRNAs were normalized to mRNA (n = 3). The expression of LHX9 in OS cell lines 143B, U2OS, MNNG-HOS and Saos-2 was determined by Western blot. hFOB.19 was the control cell line, GAPDH was used as the loading control. The relative expression of LHX9 after normalization to GAPDH was shown in the right histogram. – The result was a representative of three independent experiments. , , Error bars represented mean ± SD. values were determined by one-way analysis of variance (ANOVA) followed by Tukey post hoc test ( , ), unpaired two-tailed Students’ -test ( ) or log-rank test ( ). ** p
机译:OS组织和细胞系中的表达上调。通过RT-qPCR评估从患者切除的正常组织(实心点)和OS肿瘤组织(实心方块)中的相对表达。将mRNA水平标准化为mRNA(n = 3)。 OS患者的表达与生存之间的相关性分析。蓝线:中低表达;红线:高表达。通过RT-qPCR测量OS细胞系143B,U2OS,MNNG-HOS和Saos-2中的相对表达。 hFOB.19是对照细胞系。将mRNA标准化为mRNA(n = 3)。通过Western印迹测定LHX9在OS细胞系143B,U2OS,MNNG-HOS和Saos-2中的表达。 hFOB.19是对照细胞系,GAPDH用作上样对照。右侧直方图显示了归一化为GAPDH后LHX9的相对表达。 –结果代表了三个独立实验。 ,,误差线表示平均值±SD。数值是通过单向方差分析(ANOVA),随后的Tukey事后检验(),不成对的两尾学生检验()或对数秩检验()确定的。 ** p

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