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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Comparison of the Sodium Hydroxide Specimen Processing Method with the C18-Carboxypropylbetaine Specimen Processing Method Using Independent Specimens with Auramine Smear the MB/BacT Liquid Culture System and the COBAS AMPLICOR MTB Test
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Comparison of the Sodium Hydroxide Specimen Processing Method with the C18-Carboxypropylbetaine Specimen Processing Method Using Independent Specimens with Auramine Smear the MB/BacT Liquid Culture System and the COBAS AMPLICOR MTB Test

机译：氢氧化钠样品处理方法与C18-羧丙基甜菜碱样品处理方法的比较使用独立的标本进行金胺涂片MB / BacT液体培养系统和COBAS AMPLICOR MTB测试

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A study was performed to diagnose tuberculosis by smear, culture, and nucleic acid amplification. The study was comprised of two independent arms. Each arm used a different specimen processing method; in one arm, all specimens were processed with N-acetyl-l-cysteine-sodium hydroxide, and in the other arm, all specimens were processed with C18-carboxypropylbetaine and lytic enzymes. In each arm, all processed sediments were split for analysis by auramine smear, by culture using the MB/BacT liquid culture system and solid media, and by nucleic acid amplification using the COBAS AMPLICOR MTB test. In the N-acetyl-l-cysteine-sodium hydroxide arm, 1,468 specimens were analyzed: 65 were smear positive; 88 and 42 were culture positive for Mycobacterium tuberculosis and nontuberculous mycobacteria, respectively; and 103 were PCR positive. Relative to cultures positive for M. tuberculosis, the sensitivity and specificity of the smear were 68.2% and 99.6%, respectively, and those of PCR were 75.0% and 97.3%, respectively. In the C18-carboxypropylbetaine study arm, 1,423 specimens were analyzed: 44 were smear positive; 82 and 31 were culture positive for M. tuberculosis and nontuberculous mycobacteria, respectively; and 91 were PCR positive. The sensitivity and specificity of the smear were 48.8% and 99.7%, respectively, and those of PCR were 78.0% and 98.0%, respectively. When the two arms were compared, C18-carboxypropylbetaine specimen processing significantly increased the number of smear-negative and culture-positive specimens and significantly increased the PCR sensitivity among this same group of specimens while at the same time significantly reducing the inhibition rate.
机译：进行了一项研究，通过涂片，培养和核酸扩增来诊断结核病。该研究由两个独立的部门组成。每个手臂使用不同的标本处理方法。在一个臂中，所有标本均用N-乙酰基-1-半胱氨酸-氢氧化钠处理，而在另一臂中，所有标本均用C18-羧丙基甜菜碱和裂解酶处理。在每个分支中，将所有处理过的沉淀物进行分割，以进行金黄色胺涂片分析，使用MB / BacT液体培养系统和固体培养基进行培养以及使用COBAS AMPLICOR MTB测试进行核酸扩增。在N-乙酰基-1-半胱氨酸-氢氧化钠分支中，分析了1,468个样本：其中65个涂片阳性。 88和42分别为结核分枝杆菌和非结核分枝杆菌培养阳性；和103为PCR阳性。相对于结核分枝杆菌阳性培养物，涂片的敏感性和特异性分别为68.2％和99.6％，PCR的敏感性和特异性分别为75.0％和97.3％。在C18-羧丙基甜菜碱研究小组中，分析了1,423个样本：44个涂片阳性； 82和31分别为结核分枝杆菌和非结核分枝杆菌培养阳性；和91均为PCR阳性。涂片的敏感性和特异性分别为48.8％和99.7％，PCR的敏感性和特异性分别为78.0％和98.0％。当比较两个臂时，C18-羧丙基甜菜碱样品处理显着增加了涂片阴性和培养阳性样品的数量，并显着提高了同一组样品中的PCR敏感性，同时显着降低了抑制率。

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