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首页> 外文期刊>European journal of clinical microbiology and infectious diseases: Official publication of the European Society of Clinical Microbiology >Comparison of the Sodium Dodecyl Sulfate-Sodium Hydroxide Specimen Processing Method with the C(18)-Carboxypropylbetaine Specimen Processing Method Using the MB/BacT Liquid Culture System.
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Comparison of the Sodium Dodecyl Sulfate-Sodium Hydroxide Specimen Processing Method with the C(18)-Carboxypropylbetaine Specimen Processing Method Using the MB/BacT Liquid Culture System.

机译：使用MB / BacT液体培养系统比较十二烷基硫酸钠-氢氧化钠样品处理方法与C（18）-羧丙基甜菜碱样品处理方法的比较。

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摘要

The ability of physicians to diagnose tuberculosis is impacted by the use of smear and culture techniques combined with specimen processing methods. The objective of this study was to evaluate the effects of specimen processing on smear and culture sensitivity by comparing the specimen processing method that uses C(18)-carboxypropylbetaine with the method that combines sodium dodecyl sulfate and sodium hydroxide. A total of 1,201 specimens were entered into this study. Specimens were split approximately equally such that one-half of each specimen was processed with sodium dodecyl sulfate-sodium hydroxide, while the other half was processed with C(18)-carboxypropylbetaine. All sediments were subjected to acid-fast staining and then analyzed using the MB/BacT liquid culture system (bioMerieux, France) and solid media. The sensitivity of smear following processing with sodium dodecyl sulfate-sodium hydroxide and C(18)-carboxypropylbetaine was 61.2% and 58.6% ( P>0.05), respectively, while the specificities were identical (99.7%). The sensitivity of culture was 84.2% and 96.1% ( P<0.05), respectively. The time to detection in the MB/BacT liquid culture system was 13.2+/-5.6 and 15.0+/-8.8 days ( P>0.05), respectively, and 20.0+/-7.6 and 15.7+/-8.9 days ( P<0.05), respectively, on solid media. The contamination rates in the MB/BacT system were 0.8% and 8.7%, respectively, whereas the contamination rates on solid media were 2.6% and 4.3%, respectively. C(18)-carboxypropylbetaine specimen processing was less labor-intensive than sodium dodecyl sulfate-sodium hydroxide processing and improved the ability of laboratory staff to detect the presence of mycobacteria by culture.
机译：涂片和培养技术以及标本处理方法的结合会影响医生诊断结核病的能力。这项研究的目的是通过比较使用C（18）-羧甲基甜菜碱的标本处理方法与结合十二烷基硫酸钠和氢氧化钠的标本处理方法来评估标本处理对涂片和培养敏感性的影响。总共1,201个样本进入了这项研究。将样品大致等分，使得每个样品的一半用十二烷基硫酸钠-氢氧化钠处理，而另一半用C（18）-羧丙基甜菜碱处理。所有沉积物均经过耐酸染色，然后使用MB / BacT液体培养系统（法国bioMerieux）和固体培养基进行分析。用十二烷基硫酸钠-氢氧化钠和C（18）-羧丙基甜菜碱处理后的涂片敏感性分别为61.2％和58.6％（P> 0.05），而特异性相同（99.7％）。培养的敏感性分别为84.2％和96.1％（P <0.05）。 MB / BacT液体培养系统中的检测时间分别为13.2 +/- 5.6天和15.0 +/- 8.8天（P> 0.05），以及20.0 +/- 7.6和15.7 +/- 8.9天（P <0.05 ）分别在固态媒体上播放。 MB / BacT系统中的污染率分别为0.8％和8.7％，而固体介质的污染率分别为2.6％和4.3％。 C（18）-羧丙基甜菜碱样品处理的劳动强度低于十二烷基硫酸钠-氢氧化钠处理，并提高了实验室工作人员通过培养检测分枝杆菌的能力。

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