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Characterization of Flavonoid Compounds in Common Swedish Berry Species

机译:瑞典常见浆果中黄酮类化合物的表征

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摘要

Berries are considered an ideal source of polyphenols, especially from the flavonoid group. In this study, we examined the flavonoid content in 16 varieties of Swedish lingonberry, raspberry, blueberry, and strawberry. Nineteen flavonoids were simultaneously quantified using external standards. An additional 29 flavonoids were tentatively identified using MS as no standards were available. Quantification was done using HPLC-UV after optimization of chromatographic and extraction procedures. The method showed high linearity within the range of 2–100 μg/mL (correlation co-efficient >0.999), intra- and inter-day precision of 1.7–7.3% and average recovery above 84% for all compounds. Blueberries and lingonberries were found to contain higher contents of flavonoids (1100 mg/100 g dry weight) than raspberries and strawberries (500 mg/100 g dry weight). Anthocyanins were the dominant flavonoids in all berries. The tentatively characterized compounds contribute 18%, 29%, 61%, and 67% of the total flavonoid content in strawberries, lingonberries, raspberries, and blueberries, respectively. Overall, Swedish berries were shown to be good sources of polyphenols.
机译:浆果被认为是多酚的理想来源,尤其是来自类黄酮类。在这项研究中,我们检查了16种瑞典越橘,覆盆子,蓝莓和草莓中的类黄酮含量。使用外部标准品同时定量了19种黄酮。由于没有标准品,暂定使用MS鉴定出另外29种类黄酮。优化色谱和萃取程序后,使用HPLC-UV进行定量。该方法显示出在2–100μg/ mL范围内的高线性(相关系数> 0.999),日内和日间精度为1.7–7.3%,所有化合物的平均回收率均高于84%。发现蓝莓和越橘的黄酮含量(1100毫克/ 100克干重)比覆盆子和草莓(500毫克/ 100克干重)更高。花色苷是所有浆果中的主要类黄酮。暂定特征的化合物分别占草莓,越橘,覆盆子和蓝莓中总黄酮含量的18%,29%,61%和67%。总体而言,瑞典浆果被证明是多酚的良好来源。

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