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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Fourier-Transform Infrared Spectroscopic Analysis Is a Powerful Tool for Studying the Dynamic Changes in Staphylococcus aureus Small-Colony Variants
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Fourier-Transform Infrared Spectroscopic Analysis Is a Powerful Tool for Studying the Dynamic Changes in Staphylococcus aureus Small-Colony Variants

机译：傅里叶变换红外光谱分析是研究金黄色葡萄球菌小菌落动态变化的有力工具

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Infections due to small-colony variants (SCVs) of Staphylococcus aureus in patients with chronic and recurrent infections are an emerging problem; however, studies with this subpopulation are hampered by the fact that SCVs may exhibit unstable phenotypes, making them difficult to study, particularly in broth media. In this study, two S. aureus sets comprising the (i) normal and the (ii) SCV phenotype (clonal with normal phenotype) recovered from clinical specimens, as well as (iii) corresponding site-directed mutants displaying the SCV phenotype (knockout of hemB) and (iv) their complemented mutants were examined by Fourier-transform infrared (FTIR) spectroscopy. Phenotypes were defined on solid and in broth media. Using first-derivative infrared spectra to calculate spectral distances, hierarchical clustering based on spectral information resulted in a dendrogram with clear discrimination between SCV and normal phenotypes. The SCVs gave an FTIR fingerprint that was easily recognizable and that was much closer to other SCVs than to their parent strains. This technique offers for the first time a noninvasive approach to investigate dynamic processes of reversion of SCVs to the normal phenotype and vice versa. Thus, FTIR spectroscopy allowed a rapid and reproducible tool for the examination of different subpopulations of S. aureus on solid and in broth media for diagnostic and research purposes.
机译：在慢性和反复感染患者中，由于金黄色葡萄球菌的小菌落变种（SCV）引起的感染是一个新出现的问题。但是，SCV可能表现出不稳定的表型，这使它们难以研究，特别是在肉汤培养基中，这一事实阻碍了这种亚人群的研究。在这项研究中，从临床标本中回收了两个金黄色葡萄球菌，包括（i）正常和（ii）SCV表型（具有正常表型的克隆），以及（iii）显示SCV表型的相应定点突变体（敲除） hemB）和（iv）它们的互补突变体通过傅立叶变换红外（FTIR）光谱进行了检查。表型是在固体和肉汤培养基中定义的。使用一阶导数红外光谱计算光谱距离，基于光谱信息的层次聚类得到树状图，在SCV和正常表型之间有明显的区别。 SCV提供了易于识别的FTIR指纹，并且与其他SCV相比，其亲本菌株更近。该技术首次提供了一种非侵入性方法来研究将SCV还原为正常表型以及反之亦然的动态过程。因此，FTIR光谱学提供了一种快速且可重现的工具，用于检查固体和肉汤培养基中金黄色葡萄球菌的不同亚群，以进行诊断和研究。

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