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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Construction of a High-Density Genetic Map and Mapping of Firmness in Grapes (Vitis vinifera L.) Based on Whole-Genome Resequencing
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Construction of a High-Density Genetic Map and Mapping of Firmness in Grapes (Vitis vinifera L.) Based on Whole-Genome Resequencing

机译：基于全基因组重测序的葡萄（Vitis vinifera L.）高密度遗传图谱的构建和硬度图谱

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摘要

Berry firmness is one of the most important quality traits in table grapes. The underlying molecular and genetic mechanisms for berry firmness remain unclear. We constructed a high-density genetic map based on whole-genome resequencing to identify loci associated with berry firmness. The genetic map had 19 linkage groups, including 1662 bin markers (26,039 SNPs), covering 1463.38 cM, and the average inter-marker distance was 0.88 cM. An analysis of berry firmness in the F1 population and both parents for three consecutive years revealed continuous variability in F1, with a distribution close to the normal distribution. Based on the genetic map and phenotypic data, three potentially significant quantitative trait loci (QTLs) related to berry firmness were identified by composite interval mapping. The contribution rate of each QTL ranged from 21.5% to 28.6%. We identified four candidate genes associated with grape firmness, which are related to endoglucanase, abscisic acid (ABA), and transcription factors. A qRT-PCR analysis revealed that the expression of abscisic-aldehyde oxidase-like gene ( ) and endoglucanase 3 gene ( ) in Muscat Hamburg was higher than in Crimson Seedless at the veraison stage, which was consistent with that of parent berry firmness. These results confirmed that and are candidate genes associated with berry firmness.
机译：浆果硬度是鲜食葡萄最重要的品质特征之一。浆果硬度的潜在分子和遗传机制仍不清楚。我们基于全基因组重测序构建了高密度遗传图谱，以鉴定与浆果硬度相关的基因座。遗传图谱有19个连锁群，包括1662个bin标记（26,039个SNP），覆盖1463.38 cM，平均标记间距离为0.88 cM。连续三年对F1种群和父母双方的浆果硬度进行分析后发现，F1具有连续变异性，其分布接近正态分布。基于遗传图谱和表型数据，通过复合区间作图法鉴定了三个与浆果硬度相关的潜在重要数量性状位点（QTL）。每个QTL的贡献率从21.5％到28.6％。我们鉴定了四个与葡萄硬度相关的候选基因，它们与内切葡聚糖酶，脱落酸（ABA）和转录因子有关。 qRT-PCR分析表明，在汉堡阶段，麝香汉堡中脱落醛氧化酶样基因（）和内切葡聚糖酶3基因（）的表达高于深红无核，这与亲本浆果的硬度一致。这些结果证实了和是与浆果硬度有关的候选基因。

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