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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Comparison of Three Commercial Assays and a Modified Disk Diffusion Assay with Two Broth Microdilution Reference Assays for Testing Zygomycetes Aspergillus spp. Candida spp. and Cryptococcus neoformans with Posaconazole and Amphotericin B
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Comparison of Three Commercial Assays and a Modified Disk Diffusion Assay with Two Broth Microdilution Reference Assays for Testing Zygomycetes Aspergillus spp. Candida spp. and Cryptococcus neoformans with Posaconazole and Amphotericin B

机译：三种商业测定法和改良的圆盘扩散测定法与两种肉汤微稀释度参比测定法的比较用于测试酵母菌属曲霉菌假丝酵母​​和新型隐球菌与泊松康唑和两性霉素B的结合

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We compared posaconazole M27-A2 and M38-A MICs to Etest and YeastOne MICs for 92 zygomycetes, 126 Aspergillus isolates, 110 Candida isolates, and Cryptococcus neoformans. Reference MICs were also correlated with inhibition zone diameters in millimeters (modified M44-A disk and Neo-Sensitabs tablet methods). Etest MICs were obtained on solidified (1.5% agar) RPMI 1640 (2% dextrose), and zone diameters were obtained on supplemented (2% glucose and 0.5 μg/ml methylene blue [for all isolates]) and nonsupplemented Mueller-Hinton (MH; molds only) agar. MICs and zone diameters were obtained between 16 and 72 h. The overall agreement (% MIC pairs within a three-dilution range) between reference posaconazole and YeastOne MICs was 98 to 100% at 16 to 24 h for zygomycetes and yeasts and 99% at 24 to 48 h for Aspergillus. The overall agreement was lower between reference posaconazole and Etest MICs (94 to 97%) and by both methods with amphotericin B for all species (95 to 99.3%). For yeasts, the correlation coefficient was similar between reference posaconazole MICs and either disk (R, 0.810) or tablet (R, 0.769) zone diameter at 24 h and was superior on MH agar for molds at 16 to 48 h (R, 0.804 and 0.799 for disk and tablet, respectively). For amphotericin B, the best correlation between reference MICs and zone diameters was observed at 16 to 48 h for molds on MH agar (R, 0.736 to 0.812 and 0.765 to 0.749 for disk and tablet, respectively) and at 48 h for yeasts (R, 0.681 and 0.503 for disk and tablet, respectively). These data suggest the potential value of these alternative broth dilution and agar diffusion methods for testing posaconazole and amphotericin B in the clinical laboratory against the species evaluated.
机译：我们比较了泊沙康唑M27-A2和M38-A MIC与Etest和YeastOne MIC的92个合子菌，126个曲霉菌，110个念珠菌和新隐球菌。参考MIC也与毫米级的抑制区直径相关（改进的M44-A盘和Neo-Sensitabs片剂方法）。在凝固的（1.5％琼脂）RPMI 1640（2％葡萄糖）上获得Etest MIC，在补充的（2％葡萄糖和0.5μg/ ml的亚甲基蓝[对于所有分离物]）和未补充的Mueller-Hinton（MH）上获得区域直径；仅可用于）琼脂。在16至72小时之间获得MIC和区域直径。参考泊沙康唑和YeastOne MIC的总体一致性（在三倍稀释范围内的MIC对百分比）在16至24 h时对合子菌和酵母而言为98％至100％，对于曲霉菌在24至48 h时为99％。参考泊沙康唑和Etest MIC之间的总体一致性较低（94％至97％），并且两种方法都使用两性霉素B对所有物种而言总体一致性较低（95％至99.3％）。对于酵母，在24小时时参比泊沙康唑MIC与圆盘（R，0.810）或片剂（R，0.769）区直径之间的相关系数相似，在MH琼脂中在16至48 h时霉菌的相关系数更高（R，0.804和磁盘和平板电脑分别为0.799）。对于两性霉素B，对于MH琼脂上的霉菌（分别为圆盘和片剂，R分别为0.736至0.812和0.765至0.749）和酵母（R为48R），参比MIC与区域直径之间的最佳相关性被观察到。 ，分别为磁盘和平板电脑的0.681和0.503）。这些数据表明，这些替代的肉汤稀释法和琼脂扩散法在临床实验室中针对评估的物种测试泊沙康唑和两性霉素B的潜在价值。

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