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首页> 美国卫生研究院文献>Journal of Animal Science >PSXIII-30 Evaluation of corn silage with addition of spent substrate from cultivation of white rot mushroom.
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PSXIII-30 Evaluation of corn silage with addition of spent substrate from cultivation of white rot mushroom.

机译：PSXIII-30通过添加白腐菌培养产生的废基质评估玉米青贮饲料。

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Lignin is a compound from the cell wall of plants, associated to cellulose and hemicellulose, which reduces the action of rumen microrganisms in fiber. Some organisms, such as white rot mushroom, produce enzymes that degrade lignin with potential use to increase digestibility of fibrous feeds. This study aimed to evaluate the composition and digestibility of corn silage treated with spent substrate from cultivation of white rot mushroom, a residue rich in lignocellulolytic enzymes (lignin peroxidase, laccase, manganese peroxidase e cellulase). Corn plants were harvested, chopped and mixed to enzymes doses at 0, 10, 20 and 30 mg lignocellulolytic enzymes/kg fresh weight, and sealed in vacuum bags (4/treatment). After 60 days, silages were oven dried (55°C, 72 h) and ground in order to determine dry matter (DM), neutral detergent fiber (NDF), acid detergent fiber (ADF), crude protein (CP) and acid detergent lignin content. dry matter digestibility (IVDMD) was determined using 1 stage & method and neutral detergent fiber digestibility (IVNDFD) on ANKOM method, both with buffalo ruminal fluid. Results were analyzed using the REG procedure SAS 9.0 in regression analysis with the lowest value for Akaike Information Criterion. Increasing levels of spent substrate in corn silage resulted in a linear reduction of NDF (P=0.008), ADF (P=0.003) and lignin (P=0.002) content. The treatments did not influence CP (P>0.05). Lignin concentration decreased from 57.68 to 48.21 g/kg DM and NDF from 497.09 to 447.97 g/kg DM (compared the treatments 0 and 30). The IVDMS showed a quadratic behavior (equation y=-0.184x +5.8631x+710.22 and R =0.517) with maximum point of 756.9 g/kg of digestibility in dosage 15.9 mg lignocellulolytic enzymes and increasing levels enzymes IVNDFD presented linear increase (P=0.052). Lignocellulolytic enzymes of spent substrate from cultivation of white rot mushroom may improve the composition and digestibility of corn silage.
机译：木质素是一种来自植物细胞壁的化合物，与纤维素和半纤维素相关，可减少纤维中瘤胃微生物的作用。一些生物，例如白腐蘑菇，会产生降解木质素的酶，并有可能用于增加纤维饲料的消化率。这项研究旨在评估用白腐蘑菇培养的废基质处理的玉米青贮饲料的组成和消化率，白腐蘑菇是富含木质纤维素分解酶（木质素过氧化物酶，漆酶，锰过氧化物酶和纤维素酶）的残渣。收获玉米植物，切碎并混合至酶剂量为0、10、20和30 mg木质纤维素分解酶/ kg鲜重，并密封在真空袋中（4次/处理）。 60天后，将青贮饲料烘干（55°C，72小时）并研磨以确定干物质（DM），中性洗涤剂纤维（NDF），酸性洗涤剂纤维（ADF），粗蛋白（CP）和酸性洗涤剂木质素含量。干物质消化率（IVDMD）使用1级方法测定，中性洗涤剂纤维消化率（IVNDFD）通过ANKOM方法测定，均采用水牛瘤胃液。使用REG程序SAS 9.0在回归分析中对结果进行了分析，Akaike信息准则的价值最低。玉米青贮饲料中废底物水平的增加导致NDF（P = 0.008），ADF（P = 0.003）和木质素（P = 0.002）含量线性降低。治疗对CP无影响（P> 0.05）。木质素浓度从57.68降低至48.21 g / kg DM，NDF从497.09降低至447.97 g / kg DM（与处理0和30相比）。 IVDMS显示二次行为（方程y = -0.184x + 5.8631x + 710.22和R = 0.517），剂量为15.9 mg木质纤维素分解酶时消化率的最高点为756.9 g / kg，酶IVNDFD的水平升高呈现线性增加（P = 0.052）。白腐菌栽培中的废底物的木质纤维素分解酶可以改善玉米青贮饲料的组成和消化率。

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