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首页> 美国卫生研究院文献>Journal of Animal Science >PSXIV-20 Effect of dietary monensin on rumen fluid metabolomic profile of beef cattle.
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PSXIV-20 Effect of dietary monensin on rumen fluid metabolomic profile of beef cattle.

机译：PSXIV-20日粮莫能菌素对肉牛瘤胃液代谢组学的影响。

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Rumen fluid metabolomic analysis using ultra-performance liquid chromatography/time-of-flight mass spectrometry and univariate statistical analysis were used to evaluate the effect of dietary monensin on the rumen fluid metabolites of beef steers. Eight rumen-fistulated Holstein steers (500 – 550 kg) were used in the study for 32 d (14-d adaptation period to diet and 18-d treatment period). On day 0, steers were weighed 16 hours after feed withdrawal, stratified and blocked by initial body weight, and randomly assigned to 1 of 2 treatments: no supplementation (CON) and monensin supplementation (MON). Steers were housed in individual pens and had ad libitum access to water and red clover/alfalfa hay mixture. Corn-soybean meal-based supplement containing no or 200 mg of monensin were fed at 1 kg/steer daily. Rumen fluid was collected on the last day of the experiment. Identification of the metabolites was obtained using MS-DIAL version 2.80. Identified metabolites were merged and imported into Metaboanalyst 4.0 for univariate analysis and partial least square-discriminant analysis to identify the differential metabolites. The chemical structures of the differential metabolites were identified according to online databases such as the Human Metabolome Database, Metlin, and the Mass Bank using accurate masses and MS/MS fragments. Overall, 243 metabolites were identified. Univariate analysis, including fold change analysis and t-test, identified 12 differential metabolites which were greater ( < 0.05) in MON compared to CON. Pathway enrichment analysis showed that the differential metabolites were primarily involved in biosynthesis of arginine, proline, glycine, serine, threonine, and tryptophan and secondary metabolites. The results of this study agreed with protein-sparing effects of MON in the rumen which may probably be due to sparing of glucogenic amino acids from gluconeogenesis or to direct effects on rumen proteolysis and deamination.
机译：使用超高效液相色谱/飞行时间质谱和单变量统计分析对瘤胃液进行了代谢组学分析，以评估膳食莫能菌素对牛肉beef瘤胃液代谢产物的影响。在研究中使用了8头瘤胃-霍斯坦stein牛（500 – 550公斤），进行了3​​2天（饮食适应期14天和治疗18天）。在第0天，断奶后16小时对ste牛进行称重，根据初始体重进行分层和封闭，并随机分配给2种处理方法之一：不补充（CON）和莫能菌素补充（MON）。 ers牛放在单独的围栏中，可以随意取水和红三叶草/苜蓿干草混合物。不含或不含莫能菌素200 mg的玉米-豆粕基补充剂，每天以1 kg /头的饲养量喂养。在实验的最后一天收集瘤胃液。使用MS-DIAL 2.80版获得代谢物的鉴定。将鉴定出的代谢物合并并导入Metaboanalyst 4.0，以进行单变量分析和偏最小二乘判别分析，以鉴定差异代谢物。根据在线数据库，例如人类代谢组数据库，Metlin和Mass Bank，使用准确的质量数和MS / MS片段鉴定了差异代谢物的化学结构。总体上，鉴定出243种代谢产物。单变量分析，包括倍数变化分析和t检验，确定了12种差异代谢产物，与CON相比，MON中的代谢产物更大（<0.05）。途径富集分析表明，差异代谢物主要参与精氨酸，脯氨酸，甘氨酸，丝氨酸，苏氨酸，色氨酸和次生代谢物的生物合成。这项研究的结果与MON在瘤胃中的蛋白质保留作用相吻合，这可能是由于糖异生产生的糖原氨基酸稀少或对瘤胃蛋白水解和脱氨的直接影响。

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