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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Understanding the Molecular Epidemiology of the Footrot Pathogen Dichelobacter nodosus To Support Control and Eradication Programs
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Understanding the Molecular Epidemiology of the Footrot Pathogen Dichelobacter nodosus To Support Control and Eradication Programs

机译：了解Footrot病原菌双歧杆菌的分子流行病学以支持控制和根除计划

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The Gram-negative anaerobe Dichelobacter nodosus is the primary etiologic agent of ovine footrot. Few studies of the genetic diversity and epidemiology of D. nodosus have been done, despite the economic cost and welfare implications of the disease. This study examined a large collection of Australian isolates; 735 isolates from footrot-infected sheep from 247 farms in Western Australia (WA) were tested by pulsed-field gel electrophoresis (PFGE), and a subset of 616 isolates was tested by infrequent restriction site PCR (IRS-PCR). The genetic diversity of WA isolates was compared to that of 61 isolates from three other Australian states. WA isolates were genetically diverse, with 181 molecular types resolved by PFGE, resulting in a simple diversity ratio (SDR) of 1:4 and a Simpson's index of discrimination value (D) of 0.98. IRS-PCR resolved 77 molecular types (SDR = 1:8 and D = 0.95). The isolates were grouped into 67 clonal groups by PFGE (SDR = 1:11, D = 0.90) and 36 clonal groups by IRS-PCR (SDR = 1:17, D = 0.87). Despite the high genetic diversity, three common clonal groups predominated in WA and were found in other Australian states. On some farms, molecular type was stable over a number of years, whereas on other farms genetically diverse isolates occurred within a flock of sheep or within a hoof. This study provides a large database from which to appropriately interpret molecular types found in epidemiological investigations and to identify common and unknown types that may compromise footrot eradication or control programs.
机译：革兰氏阴性厌氧结节杆菌是绵羊脚腐病的主要病原体。尽管该病的经济成本和福利影响，但对结节藻的遗传多样性和流行病学的研究很少。这项研究检查了大量澳大利亚分离株。通过脉冲场凝胶电泳（PFGE）测试了来自西澳大利亚州（WA）247个农场的脚踏感染绵羊的735个分离株，并通过了不常见的限制性酶切位点PCR（IRS-PCR）测试了616个分离株的一个子集。比较了西澳分离株的遗传多样性与其他三个澳大利亚州的61个分离株的遗传多样性。 WA分离株具有遗传多样性，通过PFGE可以解析181种分子类型，因此简单多样性比（SDR）为1：4，辛普森鉴别指数（D）为0.98。 IRS-PCR解析了77种分子类型（SDR = 1：8和D = 0.95）。通过PFGE将分离株分为67个克隆组（SDR = 1:11，D = 0.90），通过IRS-PCR将分离株分为36个克隆组（SDR = 1:17，D = 0.87）。尽管遗传多样性高，但在西澳大利亚州仍占主导地位的三个普通克隆群在澳大利亚其他州被发现。在一些农场中，分子类型多年来保持稳定，而在其他农场中，遗传多样性分离株发生在一群羊或一个蹄内。这项研究提供了一个庞大的数据库，可以从中适当地解释流行病学调查中发现的分子类型，并确定可能危害根除或控制程序的常见和未知类型。

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