• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Multiplex 5′ Nuclease Quantitative Real-Time PCR for Clinical Diagnosis of Malaria and Species-Level Identification and Epidemiologic Evaluation of Malaria-Causing Parasites Including Plasmodium knowlesi
【2h】

Multiplex 5′ Nuclease Quantitative Real-Time PCR for Clinical Diagnosis of Malaria and Species-Level Identification and Epidemiologic Evaluation of Malaria-Causing Parasites Including Plasmodium knowlesi

机译：多重5核酸酶实时定量PCR用于疟疾的临床诊断和包括疟原虫在内的疟原虫的种级鉴定和流行病学评估

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Molecular diagnosis of malaria offers many potential advantages over microscopy, including identification of malaria to the species level in an era with few experienced microscopists. We developed high-throughput multiplex 5′ nuclease quantitative PCR (qPCR) assays, with the potential to support large studies, to specifically identify Plasmodium falciparum, P. vivax, P. ovale, P. malariae, and P. knowlesi. We compared qPCR to microscopy and confirmed discordant results with an alternative target PCR assay. The assays specifically detected 1 to 6 parasites/μl of blood. The clinical sensitivities (95% confidence intervals [CIs]) of the 4-plex assay to detect microscopically confirmed malaria were 95.8% (88.3 to 99.1%) for P. falciparum, 89.5% (75.2 to 97.1%) for P. vivax, 94.1% (71.3 to 99.9%) for P. ovale, and 100% (66.4 to 100%) for P. malariae. The specificities (95% CIs) were 98.6% (92.4 to 100%) for P. falciparum, 99% (84.8 to 100%) for P. vivax, 98.4% (94.4 to 99.8%) for P. ovale, and 99.3% (95.9 to 100%) for P. malariae. The clinical specificity for samples without malaria was 100%. The clinical sensitivity of the 5-plex assay for confirmed P. knowlesi malaria was 100% (95% CI, 69.2 to 100%), and the clinical specificity was 100% (95% CI, 87.2 to 100%). Coded retesting and testing with an alternative target PCR assay showed improved sensitivity and specificity of multiplex qPCR versus microscopy. Additionally, 91.7% (11/12) of the samples with uncertain species by microscopy were identified to the species level identically by both our multiplex qPCR assay and the alternative target PCR assay, including 9 P. falciparum infections. Multiplex qPCR can rapidly and simultaneously identify all 5 Plasmodium species known to cause malaria in humans, and it offers an alternative or adjunct to microscopy for clinical diagnosis as well as a needed high-throughput tool for research.
机译：与显微镜相比，疟疾的分子诊断具有许多潜在的优势，包括在一个经验丰富的显微镜专家很少的时代将疟疾鉴定到物种水平。我们开发了高通量多重5'核酸酶定量PCR（qPCR）分析方法，具有支持大型研究的潜力，可以特异性鉴定恶性疟原虫，间日疟原虫，卵圆形疟原虫，疟疾疟原虫和诺氏疟原虫。我们将qPCR与显微镜进行了比较，并使用替代的目标PCR测定法确认了不一致的结果。该测定法专门检测到1至6个寄生虫/微升血液。 4联检法检测恶性疟原虫的临床敏感性（95％置信区间[CIs]）对于恶性疟原虫为95.8％（88.3至99.1％），对于间日疟原虫为89.5％（75.2至97.1％），椭圆形疟原虫为94.1％（71.3至99.9％），疟疾疟原虫为100％（66.4至100％）。恶性疟原虫的特异性（95％CI）为98.6％（92.4至100％），间日疟原虫的特异性为99％（84.8至100％），卵形疟原虫的98.4％（94.4至99.8％）和99.3％ （95.9至100％）的疟疾。没有疟疾的样品的临床特异性为100％。 5-plex检测对确认的诺氏疟原虫疟疾的临床敏感性为100％（95％CI，69.2至100％），临床特异性为100％（95％CI，87.2至100％）。编码的重新检测和其他靶PCR检测方法的检测表明，与显微镜相比，多重qPCR的灵敏度和特异性有所提高。此外，通过我们的多重qPCR分析和替代性目标PCR分析，包括9种恶性疟原虫感染，通过显微镜检查，具有物种不确定性的样本中91.7％（11/12）相同地被识别到物种水平。多重qPCR可以快速，同时鉴定出已知会导致人类疟疾的所有5种疟原虫，它为临床诊断提供了显微镜的替代或辅助手段，也是研究所需的高通量工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号