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首页> 美国卫生研究院文献>Marine Drugs >Evaluation of the Antibacterial Material Production in the Fermentation of Bacillusamyloliquefaciens-9 from Whitespotted Bamboo Shark (Chiloscyllium plagiosum)
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Evaluation of the Antibacterial Material Production in the Fermentation of Bacillusamyloliquefaciens-9 from Whitespotted Bamboo Shark (Chiloscyllium plagiosum)

机译：芽孢杆菌发酵中抗菌物质产生的评价白斑竹鲨（Chiloscyllium plagiosum）的amyloliquefaciens-9

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摘要

(GBacillus-9), which is isolated from the intestinal tract of the white-spotted bamboo shark ( ), can secrete potential antibacterial materials, such as β-1,3-1,4-glucanase and some antimicrobial peptides. However, the low fermentation production has hindered the development of GBacillus-9 as biological additives. In this study, the Plackett–Burman design and response surface methodology were used to optimize the fermentation conditions in a shake flask to obtain a higher yield and antibacterial activity of GBacillus-9. On the basis of the data from medium screening, M9 medium was selected as the basic medium for fermentation. The data from the single-factor experiment showed that sucrose had the highest antibacterial activity among the 10 carbon sources. The Plackett–Burman design identified sucrose, NH Cl, and MgSO as the major variables altering antibacterial activity. The optimal concentrations of these compounds to enhance antibacterial activity were assessed using the central composite design. Data showed that sucrose, NH Cl, and MgSO had the highest antibacterial activities at concentrations of 64.8, 1.84, and 0.08 g L , respectively. The data also showed that the optimal fermentation conditions for the antibacterial material production of GBacillus-9 were as follows: Inoculum volume of 5%, initial pH of 7.0, temperature of 36 °C, rotating speed of 180 rpm, and fermentation time of 10 h. The optimal fermentation medium and conditions achieved to improve the yield of antibacterial materials for GBacillus-9 can enhance the process of developing biological additives derived from GBacillus-9.
机译：（GBacillus-9）是从白斑竹鲨（）的肠道中分离出来的，可以分泌潜在的抗菌物质，例如β-1,3-1,4-葡聚糖酶和某些抗菌肽。然而，低发酵产量阻碍了GBacillus-9作为生物添加剂的发展。在这项研究中，采用Plackett-Burman设计和响应面方法优化了摇瓶中的发酵条件，从而获得了更高的GBacillus-9产量和抗菌活性。根据培养基筛选的数据，选择M9培养基作为发酵的基本培养基。单因素实验的数据表明，蔗糖具有10种碳源中最高的抗菌活性。 Plackett-Burman设计确定了蔗糖，NH Cl和MgSO是改变抗菌活性的主要变量。使用中央复合设计评估了这些化合物增强抗菌活性的最佳浓度。数据显示，蔗糖，NH Cl和MgSO分别在64.8、1.84和0.08 g L的浓度下具有最高的抗菌活性。数据还表明，用于生产GBacillus-9抗菌材料的最佳发酵条件如下：接种量为5％，初始pH为7.0，温度为36°C，旋转速度为180 rpm，发酵时间为10 H。为提高GBacillus-9抗菌材料的产量而达到的最佳发酵培养基和条件可以增强开发源自GBacillus-9的生物添加剂的过程。

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