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首页> 美国卫生研究院文献>Molecules >Optimization of Ultrasound-Assisted Extraction Conditions Followed by Solid Phase Extraction Fractionation from Orthosiphon stamineus Benth (Lamiace) Leaves for Antiproliferative Effect on Prostate Cancer Cells
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Optimization of Ultrasound-Assisted Extraction Conditions Followed by Solid Phase Extraction Fractionation from Orthosiphon stamineus Benth (Lamiace) Leaves for Antiproliferative Effect on Prostate Cancer Cells

机译：超声辅助提取条件的优化然后对拟南芥（Lamiace）叶进行固相萃取分离以提高对前列腺癌细胞的增殖作用

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Primarily, optimization of ultrasonic-assisted extraction (UAE) conditions of was evaluated and verified using a central composite design (CCD) based on three factors including extraction time (minutes), ultrasound amplitude (A), and solvent concentration (%). The response surface methodology (RSM) was performed to develop an extraction method with maximum yield and high rosmarinic acid content. The optimal UAE conditions were as follows: extraction time 21 min, ultrasound amplitudes 62 A, and solvent composition 70% ethanol in water. The crude extract was further fractionated using solid-phase extraction (SPE), where six sequential fractions that varied in polarity (0–100% Acetonitrile in water) were obtained. Next, the six fractions were evaluated for their antioxidant and anti-cancer properties. This study found that Fraction 2 (F2) contained the highest rosmarinic acid content and showed the strongest antioxidant activity. Additionally, F2 showed an anti-proliferative effect against prostate cancer (DU145) with no harmful effect on normal cells.
机译：首先，使用中央复合设计（CCD）根据三个因素（包括提取时间（分钟），超声幅度（A）和溶剂浓度（％））评估和验证了超声辅助提取（UAE）条件的优化。进行了响应面分析（RSM），以开发一种具有最大收率和高迷迭香酸含量的提取方法。最佳的阿联酋条件如下：提取时间21分钟，超声振幅62 A，溶剂成分为70％的乙醇水溶液。使用固相萃取（SPE）进一步分离粗提物，得到六个极性连续变化的馏分（水中乙腈为0-100％）。接下来，评估这六个馏分的抗氧化和抗癌特性。该研究发现级分2（F2）含有最高的迷迭香酸含量，并显示出最强的抗氧化活性。此外，F2对前列腺癌（DU145）有抗增殖作用，对正常细胞无有害作用。

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