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Stevia Rebaudiana Bertoni a Source of High-Potency Natural Sweetener—Biochemical and Genetic Characterization

机译:甜叶菊甜叶菊高效天然甜味剂的来源—生化和遗传表征

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摘要

is a natural sweetener herb that is increasingly used in herbal medicines in the food and cosmetics industries. Molecular methods can be combined with morphological techniques to identify stevia genotypes as a starting material to produce more reliable bioproducts. This study evaluated the level of the genetic and biochemical diversity in various stevia genotypes using HPLC (high performance liquid chromatography) analysis and random amplified polymorphic DNA (RAPD) markers. Stevia genotypes collected from different locations of the world showed clear variations at the biochemical and genetic level in Polish climate conditions. The influence of the genotypes on the content of steviol glycosides, antioxidants, phenols, flavonoids, and tannins was analyzed using phytochemical assays. Genotypes from Morocco, Poland, Egypt, and Nigeria can be defined as samples of higher quality compared to other genotypes analyzed in terms of the amount of steviol glycosides. Considering the rebaudioside A/stevioside ratio as a selection criterion, genotypes from Australia, China, India, and Pakistan should be considered to be valuable in terms of suitability for obtaining new varieties. The present results of RAPD marker analysis indicated differential banding pattern and considerable polymorphism among all ten stevia genotypes. Genotypes from Morocco, Egypt, Poland, Nigeria, China, and India, as genetically different, can be selected for further stevia breeding programs.
机译:是一种天然甜味药草,越来越多地用于食品和化妆品行业的草药中。分子方法可以与形态学技术相结合,以鉴定甜叶菊基因型为起始原料,以生产更可靠的生物产品。这项研究使用HPLC(高效液相色谱)分析和随机扩增多态性DNA(RAPD)标记评估了各种甜菊基因型的遗传和生化多样性水平。在波兰的气候条件下,从世界各地采集的甜叶菊基因型在生化和遗传水平上均表现出明显的差异。基因型对甜菊糖苷,抗氧化剂,酚,类黄酮和单宁酸含量的影响采用植物化学分析法进行了分析。可以将摩洛哥,波兰,埃及和尼日利亚的基因型定义为与其他按甜菊糖苷含量分析的基因型相比质量更高的样品。考虑到莱鲍迪甙A /甜菊甙的比例作为选择标准,从澳大利亚,中国,印度和巴基斯坦的基因型来看,在获得新品种的适用性方面应该是有价值的。 RAPD标记分析的当前结果表明,在所有十种甜菊基因型之间,差异条带模式和相当多的多态性。由于遗传上的不同,可以选择来自摩洛哥,埃及,波兰,尼日利亚,中国和印度的基因型用于进一步的甜叶菊育种计划。

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