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首页> 外文学位 >Genetic and environmental control of steviol glycoside biosynthesis in Stevia rebaudiana.
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Genetic and environmental control of steviol glycoside biosynthesis in Stevia rebaudiana.

机译：甜叶菊甜菊醇糖苷生物合成的遗传和环境控制。

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摘要

Stevia rebaudiana (stevia) is gaining agricultural importance due to its natural production of non-caloric steviol glycosides, which can be extracted and incorporated into natural, zero-calorie sweeteners. Of the several steviol glycosides produced by stevia, those produced in the largest concentrations, such as rebaudioside A, are often criticized by consumers as having a bitter aftertaste. Other glycosides, such as rebaudioside D, are reported to have a superior flavor profile, but are produced in much smaller concentrations. The environmental and genetic control of steviol glycoside synthesis is poorly understood. The objectives of this research were: 1) to determine how daily light integral (DLI) influences glycoside concentrations and relative proportions, particularly for rebaudioside D and 2) to elucidate the genetic control of rebaudioside D synthesis from rebaudioside A. DLI influenced both glycoside concentration and relative proportion in a glycoside-specific manner. Using traditional breeding, populations were created via controlled crosses where parents varied in the percentage of rebaudioside D calculated from the total of rebaudiosides A and D. The production of rebaudioside D appears to be regulated by a single dominant gene controlling the presence/absence of the precursor compound, rebaudioside A. Some of the individual populations in this study exhibited correlations unique to each population regarding traits of interest, demonstrating the variability in stevia. Future breeding efforts could harness this variability in addition to knowledge of precursor glycoside content as a resource to improve future stevia lines.
机译：甜叶菊（Stevia rebaudiana（stevia））由于其天然生产的非热量甜菊醇糖苷而在农业上占有重要地位，可将其提取并掺入天然的零热量甜味剂中。甜叶菊生产的几种甜菊醇糖苷中，浓度最高的那些，如莱鲍迪苷A，经常被消费者批评为回味苦。据报道，其他糖苷，如莱鲍迪甙D，具有极好的风味，但浓度要小得多。对甜菊醇糖苷合成的环境和遗传控制知之甚少。这项研究的目的是：1）确定每日光积分（DLI）如何影响糖苷浓度和相对比例，特别是对于莱鲍迪甙D和2）阐明从莱鲍迪甙A合成莱鲍迪甙D的遗传控制。DLI影响了两种甙浓度和相对比例以糖苷特异的方式。使用传统育种，通过控制杂交产生种群，其中父母从莱鲍迪甙A和D的总和中计算出莱鲍迪甙D的百分比。前体化合物莱鲍迪甙A。在这项研究中，一些个体群体在感兴趣的性状上表现出每个群体独特的相关性，证明了甜叶菊的变异性。除了了解前体糖苷含量的知识外，未来的育种工作还可以利用这种可变性作为改进未来甜菊系的资源。

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  • 作者

    Evans, Jennifer Marie.;

  • 作者单位

    Michigan State University.;

  • 授予单位 Michigan State University.;
  • 学科 Agriculture Horticulture.;Biology Genetics.;Agriculture Plant Culture.
  • 学位 M.S.
  • 年度 2014
  • 页码 114 p.
  • 总页数 114
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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