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首页> 美国卫生研究院文献>Nanomaterials >Quantitative and Selective Surface Plasmon Resonance Response Based on a Reduced Graphene Oxide–Polyamidoamine Nanocomposite for Detection of Dengue Virus E-Proteins
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Quantitative and Selective Surface Plasmon Resonance Response Based on a Reduced Graphene Oxide–Polyamidoamine Nanocomposite for Detection of Dengue Virus E-Proteins

机译：基于减少的氧化石墨烯-聚酰胺酰胺纳米复合材料的定量和选择性表面等离子体共振响应用于检测登革热病毒电子蛋白

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Dengue viral infection is one of the most common deadliest diseases and has become a recurrent issue for public health in tropical countries. Although the spectrum of clinical diagnosis and treatment have recently been established, the efficient and rapid detection of dengue virus (DENV) during viremia and the early febrile phase is still a great challenge. In this study, a dithiobis (succinimidyl undecanoate, DSU)/amine-functionalized reduced graphene oxide-–polyamidoamine dendrimer (DSU/amine-functionalized rGO–PAMAM) thin film-based surface plasmon resonance (SPR) sensor was developed for the detection of DENV 2 E-proteins. Different concentrations of DENV 2 E-proteins were successfully tested by the developed SPR sensor-based system. The performance of the developed sensor showed increased shift in the SPR angle, narrow full-width–half-maximum of the SPR curve, high detection accuracy, excellent figure of merit and signal-to-noise ratio, good sensitivity values in the range of 0.08–0.5 pM (S = 0.2576°/pM, R = 0.92), and a high equilibrium association constant (K ) of 7.6452 TM . The developed sensor also showed a sensitive and selective response towards DENV 2 E-proteins compared to DENV 1 E-proteins and ZIKV (Zika virus) E-proteins. Overall, it was concluded that the Au/DSU/amine-functionalized rGO–PAMAM thin film-based SPR sensor has potential to serve as a rapid clinical diagnostic tool for DENV infection.
机译：登革热病毒感染是最常见的致命疾病之一，已成为热带国家公共卫生的经常性问题。尽管最近已经建立了临床诊断和治疗方法，但是在病毒血症和发热早期阶段，如何快速有效地检测登革热病毒仍然是一项巨大的挑战。在这项研究中，开发了一种基于薄膜的表面等离振子共振（SPR）传感器的二硫代双（癸酸琥珀酰胺基十一酸酯，DSU）/胺官能化的还原氧化石墨烯-聚酰胺型胺树枝状聚合物（DSU /胺官能化的rGO-PAMAM）薄膜。 DENV 2 E蛋白。通过开发的基于SPR传感器的系统成功测试了不同浓度的DENV 2 E蛋白。研发的传感器的性能表现出SPR角的偏移增加，SPR曲线的全角变窄至最大值的一半，高检测精度，优异的品质因数和信噪比，以及在0.08-0.5 pM（S = 0.2576°/ pM，R = 0.92），以及7.6452 TM的高平衡缔合常数（K）。与DENV 1 E蛋白和ZIKV（寨卡病毒）E蛋白相比，开发的传感器还显示出对DENV 2 E蛋白的敏感和选择性响应。总体而言，得出的结论是，基于Au / DSU /胺功能化的rGO–PAMAM薄膜SPR传感器具有作为DENV感染的快速临床诊断工具的潜力。

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