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首页> 美国卫生研究院文献>Engineering in Life Sciences >Development of aqueous two‐phase systems comprising poly ethylene glycol and dextran for purification of pullulan: Phase diagrams and fiscal analysis
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Development of aqueous two‐phase systems comprising poly ethylene glycol and dextran for purification of pullulan: Phase diagrams and fiscal analysis

机译：包含聚乙二醇和葡聚糖的水两相系统开发用于纯化支链淀粉：相图和财务分析

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摘要

Pullulan is a commercially important Exopolysaccharide (EPS) with wide‐spread applications which is produced by . The alternative α (1 4) & α (1 6) configuration in pullulan provides it the specific structural and conformational properties. Pullulan is currently being exploited in food, health care, pharmacy, lithography, cosmetics. The fermented broth is processed by organic solvent precipitation for isolation and purification of pullulan. In this study, we have tried to analyze the potential of aqueous two phase system as an alternate technique to extract pullulan from fermented broth. Including this viability of ATPS was also compared with conventional organic solvent precipitation system in terms of cost and time. It was found that ATPS process produced a higher yield of pullulan (80.56%) than organic solvent precipitation method (71.6%). ATPS was also found more economical and less time consuming method.
机译：普鲁兰（Pululan）是一种商业上重要的外多糖（EPS），具有广泛的应用范围，由制成。支链淀粉中的替代α（1 4）和α（1 6）构型为其提供了特定的结构和构象性质。 Pullulan目前在食品，保健，制药，光刻，化妆品中得到开发。发酵的肉汤通过有机溶剂沉淀进行处理，以分离和纯化支链淀粉。在这项研究中，我们试图分析水相两相系统作为从发酵液中提取支链淀粉的替代技术的潜力。在成本和时间方面，还包括了ATPS的这种可行性与常规有机溶剂沉淀系统的比较。结果发现，与有机溶剂沉淀法（71.6％）相比，ATPS工艺产生的支链淀粉产量更高（80.56％）。还发现ATPS更经济且耗时更少。

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