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Swift production of rhamnolipid biosurfactant biopolymer and synthesis of biosurfactant-wrapped silver nanoparticles and its enhanced oil recovery

机译:快速生产鼠李糖脂生物表面活性剂生物聚合物以及生物表面活性剂包裹的银纳米颗粒的合成及其提高的采油率

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摘要

Microbial enhanced oil recovery (MEOR) is a kind of enhanced oil recovery (EOR) development, often used as a tertiary stage where oil recovery is no longer possible utilizing primary and secondary conventional techniques. Among a few potential natural operators valuable for MEOR, biosurfactants, biopolymers and biosurfactant based nanoparticles assume key jobs. Biosurfactant which are produced by microorganisms’ act as are surface active agents that can be used as an alternative to chemically synthesized surfactants. TEN01, a gram-negative bacterium isolated from the petroleum industry is a potential biosurfactant (Rhamnolipid) producer using cassava waste as the substrate. This work focuses on production and characterization of rhamnolipid from TEN01 and its use in enhanced oil recovery. The effectiveness of Chitosan that is deacetylated form of chitin which is a biopolymer that provides density and viscosity to the fluids is not known in enhanced oil recovery yet and so it is studied. Moreover, the fabrication of biosurfactant-mediated silver nanocrystals and its application in enhanced oil recovery is also studied. Sand-Pack column was constructed and the mechanism of oil recovery in the column was studied. While incubating the crude oil containing sand packed column with Biosurfactant-biopolymer and brine flooding in the ratio of 1:2, and Biosurfactant incubation - flooding with 3 g/l of biopolymer was found to be 34.28% and 44.5% respectively. The biosurfactant based silver nanoparticles are non-toxic and have better stability when compared to chemically synthesized silver nanoparticles. The oil recovery percentage by chemical based Ag NPs and biosurfactant based Ag NPs are 14.94% and 14.28% respectively.
机译:微生物强化采油(MEOR)是一种强化采油(EOR)的开发,通常用作第三阶段,在该阶段,利用一级和二级常规技术不再可能进行采油。在一些对MEOR有价值的潜在自然操作者中,基于生物表面活性剂,生物聚合物和基于生物表面活性剂的纳米颗粒承担着重要的职责。由微生物产生的生物表面活性剂起着表面活性剂的作用,可以替代化学合成的表面活性剂。 TEN01是从石油工业中分离出来的革兰氏阴性细菌,它是利用木薯废料为底物的潜在生物表面活性剂(鼠李糖脂)生产商。这项工作着重于TEN01鼠李糖脂的生产和表征及其在提高采油率中的用途。壳聚糖是几丁质的脱乙酰基形式的壳聚糖的有效性,其是为流体提供密度和粘度的生物聚合物,但在提高采油率方面尚不知道,因此进行了研究。此外,还研究了生物表面活性剂介导的银纳米晶体的制备及其在提高采油率中的应用。构造了Sand-Pack色谱柱,研究了该色谱柱的采油机理。当用生物表面活性剂-生物聚合物和盐水驱油以1:2的比例孵育含砂填充原油的原油时,生物表面活性剂孵育-用3 g / l的生物聚合物驱油的浸出率分别为34.28%和44.5%。与化学合成的银纳米颗粒相比,基于生物表面活性剂的银纳米颗粒无毒且具有更好的稳定性。化学基Ag NP和生物表面活性剂基Ag NP的采油率分别为14.94%和14.28%。

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