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首页> 美国卫生研究院文献>Plants >Phytochemical Analysis and Establishment of Embryogenic Cell Suspension and Agrobacterium-mediated Transformation for Farmer Preferred Cultivars of West African Plantain (Musa spp.)
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Phytochemical Analysis and Establishment of Embryogenic Cell Suspension and Agrobacterium-mediated Transformation for Farmer Preferred Cultivars of West African Plantain (Musa spp.)

机译：西非车前草（Musa spp。）的农户首选栽培品种的植物化学分析以及胚性细胞悬浮和农杆菌介导的转化的建立

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摘要

Banana and plantain are among the foremost staple food crops providing food and livelihood to over 500 million people in tropical countries. Despite the importance, their production is hampered due to several biotic and abiotic stresses. Plant tissue culture techniques such as somatic embryogenesis and genetic transformation offer a valuable tool for genetic improvement. Identification and quantification of phytochemicals found in banana and plantain are essential in optimizing in vitro activities for crop improvement. Total antioxidants, phenolics, flavonoids, and tannins were quantified in various explants obtained from the field, as well as in vitro plants of banana and plantain cultivars. The result showed genotypic variation in the phytochemicals of selected cultivars. The embryogenic cell suspensions were developed for three farmer-preferred plantain cultivars, Agbagba, Obino l’Ewai, and Orishele, using different MS and B5-based culture media. Both culture media supported the development of friable embryogenic calli (FEC), while MS culture media supported the proliferation of fine cell suspension in liquid culture media. The percentage of FEC generated for Agbagba, Obino l’Ewai, and Orishele were 22 ± 24%, 13 ± 28%, and 9 ± 16%, respectively. Cell suspensions produced from FECs were successfully transformed by -mediated transformation with reporter gene constructs and regenerated into whole plants.
机译：香蕉和车前草是最主要的主粮作物，可为热带国家的5亿多人口提供粮食和生计。尽管很重要，但由于多种生物和非生物胁迫，它们的生产仍然受到阻碍。植物组织培养技术，例如体细胞胚发生和遗传转化，为遗传改良提供了宝贵的工具。鉴定和定量香蕉和车前草中发现的植物化学物质对于优化作物改良的体外活性至关重要。在从田间获得的各种外植体以及香蕉和车前草品种的体外植物中，对总抗氧化剂，酚类，类黄酮和单宁酸进行了定量。结果表明所选品种的植物化学成分具有基因型差异。使用不同的基于MS和B5的培养基，为三种农户首选的车前草品种Agbagba，Obino l'Ewai和Orishele开发了胚发生细胞悬浮液。两种培养基都支持易碎的胚性愈伤组织（FEC）的发展，而MS培养基则支持细细胞悬液在液体培养基中的增殖。 Agbagba，Obino l'Ewai和Orishele产生的FEC的百分比分别为22±24％，13±28％和9±16％。由FEC产生的细胞悬浮液通过报道基因基因构建体的介导转化而成功转化，并再生为完整植物。

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