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首页> 美国卫生研究院文献>The Journal of Clinical Investigation >Beta-adrenergic stimulation of adenine nucleotide catabolism and purine release in human adipocytes.
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Beta-adrenergic stimulation of adenine nucleotide catabolism and purine release in human adipocytes.

机译：β-肾上腺素刺激人脂肪细胞中的腺嘌呤核苷酸分解代谢和嘌呤释放。

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摘要

The effects of beta-adrenergic agonists on ATP utilization and adenine nucleotide breakdown in human adipocytes were examined. The catecholamine-induced increase in cAMP was associated with an enhancement of adenine nucleotide catabolism resulting in an increase in release of inosine and hypoxanthine which can not be reutilized for adenine nucleotide synthesis. Therefore, one-third of total cellular adenine nucleotides were irreversibly lost in the presence of 1 mumol/liter isoproterenol. The catecholamine-induced increase in purine release could be blocked by phosphodiesterase inhibitors, suggesting that cAMP is the main precursor of purines in the presence of beta-adrenergic agonists. However, epinephrine (in the simultaneous presence of the alpha 2-adrenergic blocking agent, yohimbine) and isoproterenol were 10 times more potent in stimulating purine release than in elevating cAMP. In addition, purine release ceased when cAMP was still markedly increased, suggesting a compartmentation of the cyclic nucleotide and/or involvement of the hormone-sensitive, low Km cAMP phosphodiesterase. The results document that white fat cells have an enormous potential for dissipating energy, and demonstrate that the pathway involving cAMP formation and hydrolysis constitutes the principle route of adenine nucleotide catabolism in the presence of beta-adrenergic agonists.
机译：考察了β-肾上腺素能激动剂对人脂肪细胞中ATP利用和腺嘌呤核苷酸分解的影响。儿茶酚胺诱导的cAMP的增加与腺嘌呤核苷酸分解代谢的增强有关，导致肌苷和次黄嘌呤的释放增加，而不能再用于腺嘌呤核苷酸合成。因此，在1μmol/升的异丙肾上腺素的存在下，总的细胞腺嘌呤核苷酸的三分之一不可逆地丢失。儿茶酚胺诱导的嘌呤释放增加可以被磷酸二酯酶抑制剂阻止，这表明在存在β-肾上腺素能激动剂的情况下，cAMP是嘌呤的主要前体。然而，肾上腺素（在同时存在α2-肾上腺素阻断剂育亨宾的情况下）和异丙肾上腺素在刺激嘌呤释放方面的效力比升高cAMP的效力高10倍。另外，当cAMP仍显着增加时，嘌呤的释放就停止了，这表明环核苷酸的分隔和/或激素敏感性低Km cAMP磷酸二酯酶的参与。结果表明，白色脂肪细胞具有耗散能量的巨大潜力，并证明在存在β-肾上腺素能激动剂的情况下，涉及cAMP形成和水解的途径构成了腺嘌呤核苷酸分解代谢的主要途径。

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