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Effect of Temperature Water Activity and Carbon Dioxide on Fungal Growth and Mycotoxin Production of Acclimatised Isolates of Fusarium verticillioides and F. graminearum

机译:温度水活性和二氧化碳对真菌生长和霉菌素素素富含镰刀菌和F. Graminearum的霉菌生长和霉菌毒素的影响

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摘要

Climate change is primarily manifested by elevated temperature and carbon dioxide (CO ) levels and is projected to provide suitable cultivation grounds for pests and pathogens in the otherwise unsuitable regions. The impacts of climate change have been predicted in many parts of the world, which could threaten global food safety and food security. The aim of the present work was therefore to examine the interacting effects of water activity (a ) (0.92, 0.95, 0.98 a ), CO (400, 800, 1200 ppm) and temperature (30, 35 °C and 30, 33 °C for and , respectively) on fungal growth and mycotoxin production of acclimatised isolates of and isolated from maize. To determine fungal growth, the colony diameters were measured on days 1, 3, 5, and 7. The mycotoxins produced were quantified using a quadrupole-time-of-flight mass spectrometer (QTOF-MS) combined with ultra-high-performance liquid chromatography (UHPLC) system. For , the optimum conditions for growth of fumonisin B (FB ), and fumonisin B (FB ) were 30 °C + 0.98 a + 400 ppm CO . These conditions were also optimum for growth, and zearalenone (ZEA) and deoxynivalenol (DON) production. Since 30 °C and 400 ppm CO were the baseline treatments, it was hence concluded that the elevated temperature and CO levels tested did not seem to significantly impact fungal growth and mycotoxin production of acclimatised isolates. To the best of our knowledge thus far, the present work described for the first time the effects of simulated climate change conditions on fungal growth and mycotoxin production of acclimatised isolates of and .
机译:气候变化主要由升高的温度和二氧化碳(CO)水平表现为,并预计在其他不合适的区域中为害虫和病原体提供合适的培养地。世界许多地区已经预测了气候变化的影响,可能威胁到全球食品安全和粮食安全。因此,目前工作的目的是检查水活动(A)的相互作用效果(0.92,0.95,0.98A),CO(400,800,1200ppm)和温度(30,35°C和30,33° C for and,分别为真菌生长和霉菌毒素的均匀分离含量和分离玉米的生长。为了确定真菌生长,在第1,3,5和7天测量菌落直径。使用四极 - 飞行时间质谱仪(QTOF-MS)与超高性能液体相结合定量所产生的霉菌毒素色谱(UHPLC)系统。例如,Fumonisin B(FB)和Fumonisin B(FB)生长的最佳条件为30℃+ 0.98A + 400ppm Co。这些条件也适用于生长,Zearalenone(Zea)和脱氧萘酚(Don)生产。由于30°C和400ppm Co是基线处理,因此得出结论,测试的高温和CO水平似乎似乎没有显着影响真菌生长和乳突毒素的适应性分离物的产生。迄今为止,迄今为止,本作第一次描述了模拟气候变化条件对真菌生长和霉菌毒素的影响的适应性分离株。

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