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首页> 美国卫生研究院文献>Plants >In Vitro Propagation of Isoëtes sabatina (Isoetaceae): A Key Conservation Challenge for a Critically Endangered Quillwort
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In Vitro Propagation of Isoëtes sabatina (Isoetaceae): A Key Conservation Challenge for a Critically Endangered Quillwort

机译：体外繁殖isoëtessabatina（isoetaceae）：适用于危及濒危Quillwort的关键保护挑战

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is an aquatic quillwort endemic to Italy. It is one of the rarest quillworts in Europe, and is critically endangered due to restricted range and to the continuous decline of both population and habitat quality. This study aims to develop an optimized protocol to reproduce and grow sporelings. Mature and immature megaspores were mixed with mature microspores to evaluate the influence of the developmental stage on germination and sporeling development. Two substrates, distilled water and water-agar medium, were tested for germination and sporeling emergence, and three substrates, sand, lake sediment and water-agar, were tested for transplants. A high percentage of megaspore germination (a total of 79.1%) was obtained in both substrates, higher for mature than immature spores. A total of 351 sporelings were produced in distilled water and water-agar cultures, with similar percentages (64.5% and 69.6%, respectively). The development stage of the megaspores affected both germination and sporeling development. Sporeling emergence showed significantly higher percentages in mature megaspores than immature ones (69.6% vs. 11.6%, respectively), with 85% of germinated spores developing sporelings. Only transplants over water-agar medium were successful. This protocol could be useful for the propagation of sporelings as the key step towards the planning of in situ actions to save this Mediterranean quillwort from extinction.
机译：是意大利的水生巨曲。它是欧洲最稀有的巨型曲线之一，由于狭窄的范围和人口和栖息地质量的持续下降，这是严重危害的。本研究旨在开发优化的协议，以重现和生长孢子。将成熟和未成熟的巨型孔与成熟的幼儿孢子混合，以评估发育阶段对发芽和孢子开发的影响。测试两个底物，蒸馏水和水 - 琼脂培养基，用于萌发和孢子形成，以及三种基材，沙子，湖泊沉积物和水 - 琼脂进行移植。在两种基材中获得高百分比的兆兆次萌发（总共79.1％），比未成熟的孢子更高。在蒸馏水和水 - 琼脂培养物中产生了总共351个孢子，具有相似的百分比（分别为64.5％和69.6％）。 Megaspores的发展阶段影响了萌发和孢子开发。孢子菌出苗在成熟巨型术中显示出比未成熟的巨型率显着更高（分别为69.6％，分别为11.6％），85％的发芽孢子产生孢子。只有在水琼脂培养基上移植成功。该协议可能对孢子传播的旨在使朝向原位行动规划的关键步骤，以将该地中海巨数从灭绝剥夺。

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