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methods for in vitro propagation and in vitro conservation of cells

机译:细胞体外繁殖和体外保存的方法

摘要

The propagation and/or maintenance of living cells in vitro is accomplished by attaching and growing cells on one side or surface of a hollow fiber membrane. The attached cells are propagated and/or maintained by passing oxygen through the membrane from the other side and into contact with the cells and simultaneously incubating the cells in a nutrient medium. This method is employed in tissue and cell culturing of altered and unaltered cells which attach to a surface for maintenance and growth.
机译:活细胞的体外繁殖和/或维持是通过在中空纤维膜的一侧或表面附着和生长细胞来完成的。通过使氧气从另一侧穿过膜并与细胞接触,并同时在营养培养基中孵育细胞,可以繁殖和/或维持附着的细胞。该方法用于组织和细胞培养中的,附着于表面的,经过改变和未改变的细胞,以维持和生长。

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