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首页> 美国卫生研究院文献>The Journal of Clinical Investigation >Induction of vascular endothelial tubular morphogenesis by human glioma cells. A model system for tumor angiogenesis.
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Induction of vascular endothelial tubular morphogenesis by human glioma cells. A model system for tumor angiogenesis.

机译：人神经胶质瘤细胞诱导血管内皮小管形态发生。肿瘤血管生成的模型系统。

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We have developed two different models of tumor angiogenesis by human brain tumors: one being tube formation by bovine aortic endothelial (BAE) cells cocultured with tumor cells in vitro, and other being in vivo angiogenesis in mice when tumor cells are transplanted into the dorsal sac. We investigated whether tube formation could be induced in BAE cells in type I collagen gel when these cells were cocultured with seven human glioma cell lines. Four of the seven glioma cell lines, which had high levels of basic fibroblast growth factor (bFGF) mRNA, induced tube formation by BAE cells. The tube formation was blocked by coadministration of anti-bFGF antibody. In in vivo model system of tumor angiogenesis in mice, these four cell lines were highly angiogenic. In contrast, with the other three glioma cell lines, which had poor expression of bFGF, BAE cells showed no apparent tube formation. These three cell lines did not efficiently develop capillary networks in mice. The results demonstrated a correlative relationship in the tubulogenesis of BAE cells, bFGF mRNA levels and angiogenesis in mice. The present study with two model systems of tumor angiogenesis suggests that the angiogenesis of some human glioma cell lines is mediated by bFGF, possibly via paracrine control.
机译：我们已经开发出两种不同的人脑肿瘤血管生成模型：一种是在体外与肿瘤细胞共培养的牛主动脉内皮（BAE）细胞形成管，另一种是将肿瘤细胞移植到背囊时在小鼠体内进行血管生成。 。我们研究了当将这些细胞与7种人类神经胶质瘤细胞系共培养时，是否可以在I型胶原凝胶的BAE细胞中诱导管形成。七个具有高水平碱性成纤维细胞生长因子（bFGF）mRNA的神经胶质瘤细胞系中的四个诱导BAE细胞形成管。共同施用抗bFGF抗体可阻断管的形成。在小鼠体内肿瘤血管生成的体内模型系统中，这四个细胞系是高度血管生成的。相反，对于其他三种bFGF表达较差的神经胶质瘤细胞系，BAE细胞没有明显的管形成。这三种细胞系不能在小鼠中有效地发展毛细血管网络。结果表明，在小鼠中，BAE细胞的肾小管生成，bFGF mRNA水平和血管生成具有相关性。本研究具有两个肿瘤血管生成模型系统，表明某些人类神经胶质瘤细胞系的血管生成是由bFGF介导的，可能是通过旁分泌控制的。

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