Multimerin is found in the alpha-granules of resting platelets and is synthesized by a megakaryocytic cell line.

机译：多聚体蛋白存在于静息血小板的α颗粒中并由巨核细胞系合成。

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In this report, we describe the intracellular localization of multimerin in platelets and its biosynthesis by Dami cells, a megakaryocytic cell line. Immunoelectron microscopy was used to examine frozen thin sections of resting and activated platelets. Multimerin was localized within the platelet alpha-granule in an eccentric position. Within activated platelets, multimerin was found in the surface-connected open cannalicular system and on the external plasma membrane. Light microscopic immunocytochemistry demonstrated multimerin in normal megakaryocytes and in Dami cells after stimulation with PMA. Confirmation of multimerin biosynthesis by Dami cells was obtained by metabolic labeling studies. Both platelet and Dami cell multimerin demonstrated several subunit sizes on reduced SDS-PAGE. However, peptide mapping confirmed structural homology between the different multimerin subunits. Glycosidase digestion demonstrated that multimerin is heavily glycosylated with mainly complex, N-linked carbohydrate. In contrast to the multimerin isolated from platelets, cultured Dami cells secreted mainly smaller multimers of the protein. Biosynthesis of multimerin by a megakaryocytic cell line supports endogenous biosynthesis by megakaryocytes as the origin of this platelet alpha-granule protein.

机译：在本报告中，我们描述了多聚素在血小板内的细胞内定位及其通过Dami细胞（一种巨核细胞系）的生物合成。免疫电子显微镜用于检查静止和活化血小板的冷冻薄片。 Multimerin偏心定位在血小板α颗粒内。在活化的血小板内，在表面连接的开放性小肠系统和外质膜上发现了多聚体蛋白。光学显微镜免疫细胞化学显示正常巨核细胞和Dami细胞受PMA刺激后呈多聚素。通过代谢标记研究获得了Dami细胞对多聚体蛋白生物合成的确认。血小板和Dami细胞多聚体素均在减少的SDS-PAGE上显示出多个亚基大小。但是，肽图确证了不同的多聚体蛋白亚基之间的结构同源性。糖苷酶消化表明，多聚体蛋白主要被复杂的N连接碳水化合物严重糖基化。与从血小板分离的多聚体蛋白相反，培养的Dami细胞主要分泌较小的蛋白多聚体。巨核细胞系对多聚体的生物合成支持巨核细胞作为该血小板α-颗粒蛋白的起源进行内源性生物合成。

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期刊名称 The Journal of Clinical Investigation
作者
C P Hayward; D F Bainton; J W Smith; P Horsewood; R H Stead; T J Podor; T E Warkentin; J G Kelton;
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作者单位

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年(卷),期 1993(91),6
年度 1993
页码 2630–2639
总页数 10
原文格式 PDF
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中图分类医学史;
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专利

1. Sequencing of Full-Length cDNA Encoding the alpha and beta Subunits of Human Casein Kinase II from Human Platelets and Megakaryocytic Cells. Expression of the Casein Kinase IIalpha Intronless Gene in a Megakaryocytic Cell Line. [J] . Singh LS, Kalafatis M Biochemistry . 2002,第28期

机译：编码来自人血小板和巨核细胞的人酪蛋白激酶II的α和β亚基的全长cDNA序列。酪蛋白激酶IIalpha无内含子基因在巨核细胞系中的表达。
2. Megakaryocytic cells synthesize and platelets secrete alpha5-laminins, and the endothelial laminin isoform laminin 10 (alpha5beta1gamma1) strongly promotes adhesion but not activation of platelets. [J] . Nigatu A, Sime W, Gorfu G, Thrombosis and Haemostasis: Journal of the International Society on Thrombosis and Haemostasis . 2006,第1期

机译：巨核细胞合成，血小板分泌α5-laminins，内皮层粘连蛋白同工型层粘连蛋白10（alpha5beta1gamma1）强烈促进血小板黏附，但不能活化。
3. Alpha-Granules in Resting Human Platelets Are a Spatially Clustered, Single Major Population [J] . Pokrovskaya Irina D., Storrie Brian, Yadav Shilpi, Blood: The Journal of the American Society of Hematology . 2018,第NovaelaTreatmentAppr期

机译：休息人血小板的α-颗粒是空间聚类的单一主要人口
4. Proteomic Analysis of the Platelet Membrane and Platelet Releasate: Insights into the Interaction of Platelets with other Blood Cells [C] . Geraldine M. Walsh, Daniel Ramb, Juergen Kast American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2010

机译：血小板膜和血小板的蛋白质组学分析释放：血小板与其他血细胞相互作用的见解
5. Exploring the regulation of megakaryocytic differentiation and platelet production by mechanical forces and cell-derived microparticles. [D] . Jiang, Jinlin. 2015

机译：探索通过机械力和细胞来源的微粒对巨核细胞分化和血小板产生的调节。
6. GMP-140 a platelet alpha-granule membrane protein is also synthesized by vascular endothelial cells and is localized in Weibel-Palade bodies. [O] . R P McEver, J H Beckstead, K L Moore, 1989

机译：GMP-140是一种血小板α-颗粒膜蛋白也是由血管内皮细胞合成的并且位于Weibel-Palade体中。
7. Multimerin is found in the alpha-granules of resting platelets and is synthesized by a megakaryocytic cell line. [O] . Hayward, C P, Bainton, D F, Smith, J W, 1993

机译：多聚体蛋白存在于静息血小板的α颗粒中，并由巨核细胞系合成。
8. Identification of Proteins Interacting at Blood or Plasma/Biomaterial Interfaces: Relation to Platelet and White Cell Adhesion, and to Clotting, and to Interactions Among White Cells and Platelets at Interfaces [R] . Vroman, L. , Munoz, P. C. 1981

机译：在血液或血浆/生物材料界面相互作用的蛋白质的鉴定：与血小板和白细胞粘附，凝血以及界面处白细胞和血小板之间的相互作用的关系

Multimerin is found in the alpha-granules of resting platelets and is synthesized by a megakaryocytic cell line.

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