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首页> 美国卫生研究院文献>Genes >A New Intra-Specific and High-Resolution Genetic Map of Eggplant Based on a RIL Population and Location of QTLs Related to Plant Anthocyanin Pigmentation and Seed Vigour
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A New Intra-Specific and High-Resolution Genetic Map of Eggplant Based on a RIL Population and Location of QTLs Related to Plant Anthocyanin Pigmentation and Seed Vigour

机译：基于RIL群体的茄子的新的特异性和高分辨率遗传图谱以及与植物花青素色素沉着和种子活力有关的QTL的位置

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Eggplant is the second most important solanaceous berry-producing crop after tomato. Despite mapping studies based on bi-parental progenies and GWAS approaches having been performed, an eggplant intraspecific high-resolution map is still lacking. We developed a RIL population from the intraspecific cross ‘305E40’, (androgenetic introgressed line carrying the locus conferring resistance) x ‘67/3’ (breeding line whose genome sequence was recently released). One hundred and sixty-three RILs were genotyped by a genotype-by-sequencing (GBS) approach, which allowed us to identify 10,361 polymorphic sites. Overall, 267 Gb of sequencing data were generated and ~773 M Illumina paired end (PE) reads were mapped against the reference sequence. A new linkage map was developed, including 7249 SNPs assigned to the 12 chromosomes and spanning 2169.23 cM, with iaci@liberoan average distance of 0.4 cM between adjacent markers. This was used to elucidate the genetic bases of seven traits related to anthocyanin content in different organs recorded in three locations as well as seed vigor. Overall, from 7 to 17 QTLs (at least one major QTL) were identified for each trait. These results demonstrate that our newly developed map supplies valuable information for QTL fine mapping, candidate gene identification, and the development of molecular markers for marker assisted selection (MAS) of favorable alleles.
机译：茄子是番茄后第二个最重要的孤独浆果作物。尽管基于双亲子生物的映射研究和已经进行了GWAS方法，但仍然缺乏茄子的拆散高分辨率地图。我们开发了来自intrachecific交叉'305e40'的RIL群体，（携带基因座赋予抵抗的基因座的血管渗透性线）X'67 / 3'（最近释放的基因组序列的繁殖线）。一百六十三个rils是通过逐序列（GBS）方法进行基因分型，这使我们允许我们鉴定10,361个多态性位点。总体而言，生成267 GB的测序数据，并映射〜773M Illumina成对端（PE）读取映射参考序列。开发了一种新的联系地图，包括分配给12染色体的7249个SNP，跨越2169.23厘米，在相邻标记之间的IACI @ Liberoan平均距离为0.4厘米。这用于阐明与在三个地点的不同器官和种子活力中的不同器官中有七种与花青素含量相关的遗传基因。总的来说，从7到17个QTL（至少一个主要QTL）被确定为每个特征。这些结果表明，我们的新开发的地图为QTL精细映射，候选基因鉴定和分子标记的显影提供了有利等位基因的标记辅助选择（MAS）的显着信息。

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