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首页> 美国卫生研究院文献>Aging (Albany NY) >The effect of Ganoderma lucidum spore oil in early skin wound healing: interactions of skin microbiota and inflammation
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The effect of Ganoderma lucidum spore oil in early skin wound healing: interactions of skin microbiota and inflammation

机译：灵芝孢子油在早期皮肤伤口愈合中的作用：皮肤微生物肿瘤的相互作用和炎症

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The mushroom has been a traditional Chinese medicine for millennia. In this study, we isolated the spore oil (GLSO) and evaluated the effect of GLSO on skin burn wound healing and the underlying mechanisms. Mice were used to perform skin wound healing assay. Wound analysis was performed by photography, hematoxylin/eosin staining, Masson’s Trichrome staining and immunohistochemical analysis. Microbiota on the wounds were analyzed using the 16s rRNA sequence and quantitative statistics. The lipopolysaccharide (LPS) content was examined in skin wounds and serum using an enzyme-linked immunosorbent assay (ELISA). The expression of Toll-like receptor 4 (TLR4) and the relative levels of inflammatory cytokines were determined by qPCR and immunofluorescence assay. A pseudo-germfree mouse model treated with antibiotics was used to investigate whether GLSO accelerated skin burn wound healing through the skin microbiota. We found that GLSO significantly accelerated the process of skin wound healing and regulated the levels of gram-negative and gram-positive bacteria. Furthermore, GLSO reduced LPS and TLR4, and levels of some other related inflammatory cytokines. The assay with the pseudo-germfree mice model showed that GLSO had a significant acceleration on skin wound healing in comparison with antibiotic treatment. Thus, GLSO downregulated the inflammation by regulating skin microbiota to accelerate skin wound healing. These findings provide a scientific rationale for the potential therapeutic use of GLSO in skin burn injury.
机译：蘑菇是千年中的中药。在这项研究中，我们分离孢子油（GLSO）并评估了GLSO对皮肤烧伤伤口愈合和潜在机制的影响。使用小鼠进行皮肤伤口愈合测定。伤口分析通过摄影，苏木精/曙红染色，Masson的三色染色和免疫组化分析进行。使用16S rRNA序列和定量统计分析伤口上的微生物。使用酶联免疫吸附试验（ELISA）在皮肤伤口和血清中检查脂多糖（LPS）含量。通过QPCR和免疫荧光测定法测定Toll样受体4（TLR4）的表达和炎性细胞因子的相对水平。用抗生素治疗的假细胞小鼠模型用于研究GRSO是否会加速皮肤烧伤伤口愈合通过皮肤微生物。我们发现GLSO显着加速了皮肤伤口愈合的过程，并调节了革兰氏阴性和革兰氏阳性细菌的水平。此外，GLSO降低了LPS和TLR4，以及一些其他相关炎症细胞因子的水平。与伪细菌模型的测定模型表明，与抗生素治疗相比，GLSO对皮肤伤口愈合具有显着的加速。因此，GLSO通过调节皮肤微生物群来加速皮肤伤口愈合来下调炎症。这些发现提供了一种科学理论，用于在皮肤灼伤损伤中潜在的治疗使用。

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