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β-Carotene-Induced Alterations in Haemoglobin Affinity to O

机译：β-胡萝卜素诱导血红蛋白亲和力的改变

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β-Carotene (β-Crt) can be dispersed in hydrophobic regions of the membrane of red blood cells (RBC). Its location, orientation and distribution strongly depend on carotenoid concentration. In the present pilot trial (six human subjects involved), it is demonstrated that incubation of RBCs with β-Crt (1.8 × 107 β-Crt molecules per RBC, 50 μmol/L) results in expansion of the membrane of RBCs and slight elongation of the cell. The changes are of statistical significance, as verified by the Wilcoxon test at p < 0.05. They indicate (i) a highly random orientation and location of β-Crt inside the membrane and (ii) a tendency for its interaction with membrane skeleton proteins. The accompanying effect of decreased RBC resistance to lysis is possibly a result of the incorrect functioning of ion channels due to their modification/disruption. At higher β-Crt concentrations, its clustering inside membranes may occur, leading to further alterations in the shape and size of RBCs, with the most pronounced changes observed at 1.8 × 108 β-Crt molecules per RBC (500 μmol/L). Due to the reduced permeability of ions, such membranes exhibit increased resistance to haemolysis. Finally, we show that interactions of β-Crt with the membrane of RBCs lead to an alteration in haemoglobin-oxygen affinity, shifting the oxyhaemoglobin dissociation curve toward higher oxygen partial pressures. If the impact of β-Crt on a curve course is confirmed in vivo, one may consider its role in the fine tuning of O2 transportation to tissues. Hence, at low concentrations, providing unchanged elastic and functional properties of RBCs, it could serve as a beneficial agent in optimising heart performance and cardiovascular load.
机译：β-胡萝卜素（β-CRT）可以分散在红细胞膜（RBC）的疏水区域中。它的位置，方向和分布强烈依赖于类胡萝卜素浓度。在目前的试验试验中（涉及六种人类受试者）中，证明RBC与β-CRT（1.8×107β-CRT分子为每RBC，50μmol/ L）孵育导致RBCS膜的膨胀和轻微的伸长率细胞。该变化具有统计学意义，如P <0.05的氟硅试验所验证。它们表明（i）膜内的高随机取向和β-CRT的位置和（ii）与膜骨架蛋白相互作用的趋势。降低RBC抗裂解的伴随效果可能是由于其改性/破坏而导致离子通道不正确的结果。在较高的β-CRT浓度下，其内部膜内的聚类可能发生，导致RBC的形状和大小的进一步改变，并且在每RBC（500μmol/ L）下观察到的1.8×108β-CRT分子中最明显的变化。由于离子的渗透性降低，这种膜表现出增加的抗溶血性。最后，我们表明β-CRT与RBC膜的相互作用导致血红蛋白 - 氧亲和力的改变，使氧气血红蛋白离解曲线变化朝向更高的氧气压力。如果在体内确认β-CRT在曲线过程中的影响，则可以考虑其在O2运输的微调中的作用。因此，在低浓度下，提供RBC的不变弹性和功能性，它可以作为优化心脏性能和心血管载荷的有益剂。

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