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Thermo-responsive imprinted hydrogel with switchable sialic acid recognition for selective cancer cell isolation from blood

机译：具有可切换唾液酸识别的热响应式压印水凝胶用于血液的选择性癌细胞分离

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摘要

In this work, a sialic acid (SA)-imprinted thermo-responsive hydrogel layer was prepared for selective capture and release of cancer cells. The SA-imprinting process was performed at 37 °C using thermo-responsive functional monomer, thus generating switchable SA-recognition sites with potent SA binding at 37 °C and weak binding at a lower temperature (e.g., 25 °C). Since SA is often overexpressed at the glycan terminals of cell membrane proteins or lipids, the SA-imprinted hydrogel layer could be used for selective cancer cell recognition. Our results confirmed that the hydrogel layer could efficiently capture cancer cells from not only the culture medium but also the real blood samples. In addition, the captured cells could be non-invasively released by lowing the temperature. Considering the non-invasive processing mode, considerable capture efficiency, good cell selectivity, as well as the more stable and durable SA-imprinted sites compared to natural antibodies or receptors, this thermo-responsive hydrogel layer could be used as a promising and general platform for cell-based cancer diagnosis.
机译：在这项工作中，制备唾液酸（SA）-Imprinted热响应水凝胶层以选择性捕获和释放癌细胞。使用热响应官能单体在37℃下在37℃下进行SA印迹方法，从而在37℃下产生有效SA结合的可切换SA识别位点，在较低温度（例如，25℃）下弱结合。由于SA通常在细胞膜蛋白或脂质的聚糖末端过表达，因此SA印迹水凝胶层可用于选择性癌细胞识别。我们的结果证实，水凝胶层可以有效地从培养基中有效地捕获癌细胞，也可以有效地捕获癌细胞。另外，通过降低温度，可以不侵入地释放捕获的细胞。考虑到非侵入性加工模式，与天然抗体或受体相比，相当苛性的捕获效率，良好的细胞选择性，以及更稳定和耐用的SA印迹位点，该热响应水凝胶层可用作有前途和一般平台用于基于细胞的癌症诊断。

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