• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Communications Biology >A combinatorial action of GmMYB176 and GmbZIP5 controls isoflavonoid biosynthesis in soybean (
【2h】

A combinatorial action of GmMYB176 and GmbZIP5 controls isoflavonoid biosynthesis in soybean (

机译：Gmmyb176和Gmbzip5的组合作用对等大豆（甲基）对等异黄酮生物合成进行控制（

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

a Subcellular localization of GmMYB176-YFP and YFP-GmMYB176 in hairy roots. Both GmMYB176-YFP and YFP-GmMYB176 fusion proteins were localized in the nucleus and the cytoplasm of soybean hairy root cells as observed by confocal microscopy. Scale bar = 50 μm. b Crude protein extracts were subjected for Co-IP assay using anti-GFP microbeads and µMAC epitope tag protein isolation system. Samples from each step were separated on an SDS-PAGE and visualized by silver staining (top gel). The bottom image shows Western blot analysis using anti-GFP monoclonal antibody. The arrow indicates the estimated size of GmMYB176-YFP protein in the eluate. Crude: crude protein extract from soybean hairy roots; Flow through: crude extract incubated with anti-GFP microbeads and applied to μcolumn, with the flow through collected; Wash: sequential wash steps with lysis buffer; Eluate: elution of bound proteins from the column; –ve control: crude extract from control hairy roots. c Venn diagram showing the overlap of GmMYB176-YFP, YFP-GmMYB176, and YFP-only interacting candidate proteins in soybean hairy roots identified by LC–MS/MS analysis. The YFP interacting protein candidates were obtained from our previous study21. d ‘GO’ annotations of the 716 candidate GmMYB176-interacting proteins. List of soybean genes encoding the candidate proteins was used in PhytoMine22 to generate annotations regarding the biological process, cellular component, and the molecular function of the candidates.
机译：GMMYB176-YFP和YFP-GMMYB176在毛状根的亚细胞定位。 GMMYB176-YFP和YFP-GMMYB176融合蛋白在核中局部化，如通过共聚焦显微镜观察到的大豆毛状根细胞的细胞质。秤条=50μm。 B粗蛋白质提取物使用抗GFP微珠和μmac表位标签蛋白质分离系统进行共同IP测定。将来自每个步骤的样品在SDS-PAGE上分离并由银染色（顶凝胶）可视化。底部图像显示使用抗GFP单克隆抗体的Western印迹分析。箭头表示洗脱液中GMMYB176-YFP蛋白的估计大小。原油：来自大豆毛的粗蛋白提取物;流过：用抗GFP微珠孵育原油提取物并施加到μCOLUMN，流过流动;洗涤：用裂解缓冲液的顺序洗涤步骤;洗脱液：从柱中洗脱结合蛋白质; -VE控制：来自控制毛根的原油提取物。 C Venn图显示GMMyB176-YFP，YFP-GMMYB176和仅由LC-MS / MS分析鉴定的大豆毛根中的YFP相互作用蛋白质的重叠。 YFP相互作用的蛋白候选者是从我们以前的研究21获得的。 D'GO'注释716候选GMMYB176相互作用蛋白。编码候选蛋白质的大豆基因列表用于植物霉素22以产生关于生物过程，细胞组分和候选物的分子功能的注释。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号