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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Early Detection of Cartilage Degeneration: A Comparison of Histology Fiber Bragg Grating-Based Micro-Indentation and Atomic Force Microscopy-Based Nano-Indentation
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Early Detection of Cartilage Degeneration: A Comparison of Histology Fiber Bragg Grating-Based Micro-Indentation and Atomic Force Microscopy-Based Nano-Indentation

机译：软骨变性的早期检测：组织学基于纤维纤维的微压缩和基于原子力显微镜的纳米凹口的比较

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We have determined the sensitivity and detection limit of a new fiber Bragg grating (FBG)-based optoelectronic micro-indenter for biomechanical testing of cartilage and compared the results to indentation-type atomic force microscopy (IT-AFM) and histological staining. As test samples, we used bovine articular cartilage, which was enzymatically degraded ex vivo for five minutes using different concentrations of collagenase (5, 50, 100 and 500 µg/mL) to mimic moderate extracellular matrix deterioration seen in early-stage osteoarthritis (OA). Picrosirius Red staining and polarization microscopy demonstrated gradual, concentration-dependent disorganization of the collagen fibrillar network in the superficial zone of the explants. Osteoarthritis Research Society International (OARSI) grading of histopathological changes did not discriminate between undigested and enzymatically degraded explants. IT-AFM was the most sensitive method for detecting minute changes in cartilage biomechanics induced by the lowest collagenase concentration, however, it did not distinguish different levels of cartilage degeneration for collagenase concentrations higher than 5 µg/mL. The FBG micro-indenter provided a better and more precise assessment of the level of cartilage degeneration than the OARSI histological grading system but it was less sensitive at detecting mechanical changes than IT-AFM. The FBG-sensor allowed us to observe differences in cartilage biomechanics for collagenase concentrations of 100 and 500 µg/mL. Our results confirm that the FBG sensor is capable of detecting small changes in articular cartilage stiffness, which may be associated with initial cartilage degeneration caused by early OA.
机译：我们已经确定了新的光纤布拉格光栅（FBG）的光电子微型压痕的灵敏度和检测限，用于软骨的生物力学测试，并将结果与​​压痕型原子力显微镜（IT-AFM）和组织学染色进行比较。作为试验样品，我们使用牛关节软骨，其使用不同浓度的胶原酶（5,50,100和500μg/ ml）酶促降解了5分钟，以模仿早期骨关节炎（OA）中观察到的中度细胞外基质劣化（OA ）。 Picrosirius红染色和偏振显微镜显示出在外植体的浅表区中胶原纤维网的逐渐依赖性依赖性紊乱。骨关节炎研究会国际（Oarsi）的组织病理学变化的分级并未区分未消化和酶促降解的外植体。 IT-AFM是检测由最低胶原酶浓度诱导的软骨生物力学中的微小变化的最敏感的方法，但是，它没有区分胶原酶浓度高于5μg/ mL的不同水平的软骨变性。 FBG微肾上腺素提供了比Oarsi组织学分级系统更好，更精确地评​​估软骨变性水平，但它在检测到比IT-AFM的机械变化不太敏感。 FBG传感器允许我们观察胶原酶浓度为100和500μg/ ml的软骨生物力学的差异。我们的结果证实，FBG传感器能够检测关节软骨刚度的小变化，这可能与早期OA引起的初始软骨变性相关。

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