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首页> 美国卫生研究院文献>Synthetic and Systems Biotechnology >The Fold-Illuminator: A low-cost portable and disposable incubator-illuminator device
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The Fold-Illuminator: A low-cost portable and disposable incubator-illuminator device

机译：折灯照明器：低成本便携式和一次性培养箱 - 发光器装置

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Fluorescent reporters have revolutionized modern applications in the fields of molecular and synthetic biology, enabling applications ranging from education to point-of-care diagnostics. Past advancements in these fields have primarily focused on improving reaction conditions, the development of new applications, and the broad dissemination of these technologies. However, field and classroom-based applications have remained limited in part due to the nature of fluorescent signal detection, which often requires the use of costly lab equipment to observe and quantify fluorescence readouts. Users without access to laboratory equipment rely on qualitative assessments of fluorescence, a process that remains highly variable from user-to-user even within the same classroom. To overcome this challenge, we have developed a foldable illuminator and incubator device to support field-applications of synthetic biology-based biosensors for education and diagnostics. The Fold-Illuminator is an affordable, portable, and recyclable device that allows for the visible detection of fluorescent biomolecules. The Fold-Illuminator's design allows for assembly in under 10 min, a user can then utilize the optional heating element to incubate biochemical reactions and visualize fluorescence outputs in a defined and light-controlled environment. Interchangeable LED strips and light-filtering screens provide modularity to pair with the fluorescence wavelengths of interest. The user can then unfold the device for convenient storage, transport, or even recycling. The cost for the Fold-Illuminator is $5.58 USD and is compatible with an optional heating element for an additional $3.98 cost, with potential for further reductions in cost for larger quantities. Open-source templates for cutting device parts from paper stock are provided for both printing and cutting by hand; cutting can also be achieved with consumer-grade smart cutting machines such as the Cricut®. Combined with the broad applications of fluorescent reporters, the Fold-Illuminator has the potential to improve access to fluorescence visualization and quantification for new users as well as emerging field applications.
机译：荧光记者在分子和合成生物学领域彻底改变了现代应用，使应用能够从教育到护理点诊断。这些领域的过去的进步主要集中在改善反应条件，新应用的发展以及这些技术的广泛传播。然而，由于荧光信号检测的性质，基于领域和基于课堂的应用程序仍然有限，这通常需要使用昂贵的实验室设备观察和量化荧光读数。用户无权依赖于实验室设备的荧光评估，即使在同一教室内也是用户到用户身上仍然变化的过程。为了克服这一挑战，我们开发了一种可折叠的照明器和培养箱设备，以支持用于教育和诊断的合成生物学的生物传感器的现场应用。折叠照明器是一种经济实惠的便携式和可回收装置，其允许荧光生物分子的可见检测。折叠照明器的设计允许在10分钟内组装，然后使用者可以利用可选的加热元件来孵育生化反应，并在定义和光控制的环境中可视化荧光输出。可互换的LED条和光滤网提供模块化与感兴趣的荧光波长配对。然后，用户可以展开设备以方便地存储，运输甚至回收。折叠式发光器的成本为5.58美元，并与可选的加热元件兼容，额外的价格为3.98美元，具有进一步降低成本的巨额。提供用于切割纸张零件的开源模板，用于手动打印和切割;也可以通过诸如CRICUT®等消费级智能切割机来实现切割。结合荧光报道器的广泛应用，折叠照明器有可能改善对新用户的荧光可视化和量化以及新兴的现场应用。

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