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Characterization of Signal Sequences Determining the Nuclear/Nucleolar Import and Nuclear Export of the Caprine Arthritis-Encephalitis Virus Rev Protein

机译：确定核/核聚体进口和核炎群关节炎 - 脑炎病毒Rev蛋白的核/核聚体进口核出口的信号序列的表征

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摘要

Caprine arthritis-encephalitis virus (CAEV), a lentivirus, relies on the action of the Rev protein for its replication. The CAEV Rev fulfills its function by allowing the nuclear exportation of partially spliced or unspliced viral mRNAs. In this study, we characterized the nuclear and nucleolar localization signals (NLS and NoLS, respectively) and the nuclear export signal (NES) of the CAEV Rev protein. These signals are key actors in the nucleocytoplasmic shuttling of a lentiviral Rev protein. Several deletion and alanine substitution mutants were generated from a plasmid encoding the CAEV Rev wild-type protein that was fused to the enhanced green fluorescent protein (EGFP). Following cell transfection, images were captured by confocal microscopy and the fluorescence was quantified in the different cell compartments. The results showed that the NLS region is localized between amino acids (aa) 59 to 75, has a monopartite-like structure and is exclusively composed of arginine residues. The NoLS was found to be partially associated with the NLS. Finally, the CAEV Rev protein’s NES mapped between aa 89 to 101, with an aa spacing between the hydrophobic residues that was found to be unconventional as compared to that of other retroviral Rev/Rev-like proteins.
机译：Caprine关节炎 - 脑炎病毒（CAEV），一种慢病毒，依赖于Rev蛋白的作用进行复制。 CAEV REV通过允许部分剪接或未甲病毒MRNA的核出口来实现其功能。在本研究中，我们以CAEV转速蛋白的核和核聚体定位信号（分别为核毒性信号（NES）和核导出信号（NES）。这些信号是慢病毒Rev蛋白的核细胞型梭中的关键actor。从编码CAEV Rev野生型蛋白质的质粒产生几种缺失和丙氨酸取代突变体，该蛋白质与增强的绿色荧光蛋白（EGFP）融合。在细胞转染后，通过共聚焦显微镜捕获图像，并在不同的细胞室中定量荧光。结果表明，NLS区域在氨基酸（AA）59至75之间定位，具有单颗粒状结构，并且专门由精氨酸残基组成。发现NOLS与NLS部分相关。最后，CAEV Rev蛋白的NE在AA 89至101之间映射，疏水残留物之间的AA间距，与其他逆转录病毒Rev / Rev样蛋白相比，发现是非常规的。

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