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Reagents and methods for detection and characterization of protein-protein interactions, nuclear export and localization sequences and inducible Gal4p-mediated gene expression in yeast
Reagents and methods for detection and characterization of protein-protein interactions, nuclear export and localization sequences and inducible Gal4p-mediated gene expression in yeast
This invention provides methods for cytoplasmic detection of protein—protein interactions, nuclear export/localization sequences, and galactose-independent inducible Gal4p-mediated gene expression through the utilization of GAL regulatory factor, Gal80p, and the yeast galactose regulon.
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机译:本发明提供了通过利用GAL调节因子Gal80 p利用细胞质检测蛋白质-蛋白质相互作用,核输出/定位序列以及不依赖半乳糖的诱导型Gal4 p I>介导的基因表达的方法。 I>和酵母半乳糖调节剂。
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