a Cartoon of recording configuration with loose-patch, single-unit recording in primary somatosensory cortex in combination with high-speed videography of self-induced whisker use and object touch. b Series of single frames from high-speed videography to illustrate offline analysis of whisker position (Θ) and object touch at 5 ms resolution. c Population statistics for duration of a whisker touch, the gap between touch end and consecutive touch start, and cycle, which is the time between consecutive touch starts. Boxplots show median as central mark, the edges of the box the 25th and 75th percentiles, the whiskers extend to the most extreme data points. Outliers are omitted for clarity. d Neurolucida reconstruction of an example L5 slender-tufted pyramid (L5st) and average (regular) AP waveform. Scale bar inset: 4 mV, 1 ms. e Loose-patch recording of the morphologically identified L5st showing the continuous voltage trace (black), extracted APs (ticks), and offline tracked whisker position (gray) with object touches highlighted by blue bars and gray shading. f Raster plot with AP spiking aligned to consecutive touches. g Peri-stimulus-time-histogram (PSTH) aligned to touch start. h–k Analogous to d–g but for L5 thick-tufted pyramid (L5tt). Note the cell-type-specific spiking response upon whisker touch (g versus k).
展开▼
