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首页> 美国卫生研究院文献>Microbiology Insights >Isolation and Characterization of a Bacterial Strain Enterobacter cloacae (Accession No. KX438060.1) Capable of Degrading DDTs Under Aerobic Conditions and Its Use in Bioremediation of Contaminated Soil
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Isolation and Characterization of a Bacterial Strain Enterobacter cloacae (Accession No. KX438060.1) Capable of Degrading DDTs Under Aerobic Conditions and Its Use in Bioremediation of Contaminated Soil

机译：细菌菌株肠杆菌的分离与表征能够降解DDTS在有氧条件下降解DDT及其在受污染土壤生物修复下的影响

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DDT is one of the most persistent pesticides among all the different types of organo-chlorine pesticides used. Among all the degradation methods, bacterial degradation of DDT is most effective. The present study was conducted to isolate different bacteria present in waste samples which have the ability to degrade DDT present in the soil in the minimum possible period of time and to observe the effect of different physical and chemical properties of the soil samples. Many pesticide degrading bacteria were isolated and identified through cultural, biochemical tests and further identified by 16S RNA sequencing method. The most potent strain DDT 1 growth in mineral salt medium supplemented with DDT as the only source of carbon (5-100 PPM) and was monitored at an optical density of 600 nm. The growth parameters at different physio-chemical conditions were further optimized. The result showed that Enterobacter cloacae had maximum growth in 15 days. FTIR analysis of the residual DDT after 15 days incubation showed that Enterobacter cloacae was able to degrade pesticide into its further metabolites of DDD, DDE, DDNU and other components can be used for biodegradation of DDT present in contaminated soil and water ecosystems.
机译：DDT是所使用的所有不同类型有机氯杀虫剂中最持久的杀虫剂之一。在所有降解方法中，DDT的细菌降解最有效。进行本研究以分离废物中存在的不同细菌，其具有在最低可能的时间内降解土壤中存在的DDT，并观察土壤样品的不同物理和化学性质的影响。分离许多农药降解细菌，并通过文化，生化试验鉴定并进一步鉴定为16S RNA测序方法。最有效的菌株DDT 1在补充有DDT的矿物盐培养基中的生长，作为唯一的碳源（5-100ppm），并以600nm的光密度监测。进一步优化了不同物理化学条件下的生长参数。结果表明，肠杆菌在15天内最大增长。孵育15天后残留DDT的FTIR分析表明，肠易裂解物能够将农药降解到其进一步的DDD，DDE，DDNU和其他组分中可用于污染的土壤和水生态系统中DDT的生物降解。

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