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首页> 外文期刊>Journal of environmental science and health >Isolation and characterization of an Alcaligenes sp. strain DG-5 capable of degrading DDTs under aerobic conditions
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Isolation and characterization of an Alcaligenes sp. strain DG-5 capable of degrading DDTs under aerobic conditions

机译：Alcaligenes sp。的分离和鉴定。需氧条件下能降解滴滴涕的DG-5菌株

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摘要

In this study, an Alcaligenes sp. strain DG-5 that can effectively degrade dichlorodiphenyltrichloro-ethanes (DDTs) under aerobic conditions was isolated from DDTs-contaminated sediment. Various factors that affect the biodegradation of DDTs by DG-5 were investigated. About 88 %, 65 % and 45 % of the total DDTs were consumed within 120 h when their initial concentrations were 0.5, 5 and 15 mg L~(-1), respectively. However, almost no degradation was observed when their concentration was increased to 30 mg L~', but the addition of nutrients significantly improved the degradation, and 66 % and 90 % of the total DDTs were degraded at 336 h in the presence of 5 g L~(-1) peptone and yeast extract, respectively. Moreover, the addition of 20 mM formate also enhanced the ability of DG-5 to transform DDTs, and its DDT transformation capacity (Tc) value was increased by 1.8 - 2.7 fold for the pure (p,p'-DDT or o,p'-DDT only) and mixed systems (p,p'-DDT, o,p'-DDT, p,p'-DDD and p,p'-DDE). Furthermore, it was found that competitive inhibition in the biodegradation by DDT compounds occurred in the mixed system.
机译：在这项研究中，Alcaligenes sp.。从DDTs污染的沉积物中分离出可以在有氧条件下有效降解二氯二苯基三氯乙烷（DDTs）的DG-5菌株。研究了影响DG-5对DDT生物降解的各种因素。当其初始浓度分别为0.5、5和15 mg L〜（-1）时，在120小时内消耗了全部DDT的约88％，65％和45％。但是，当其浓度增加到30 mg L〜'时，几乎没有观察到降解，但是添加营养物质显着改善了降解，在5 g存在下，在336 h降解了总DDT的66％和90％。 L〜（-1）蛋白ept和酵母提取物。此外，添加20 mM甲酸盐还增强了DG-5转化DDT的能力，对于纯净（p，p'-DDT或o，p），其DDT转化能力（Tc）值提高了1.8-2.7倍。仅限'-DDT）和混合系统（p，p'-DDT，o，p'-DDT，p，p'-DDD和p，p'-DDE）。此外，发现在混合系统中发生了DDT化合物对生物降解的竞争性抑制。

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  • 来源
    《Journal of environmental science and health》 |2011年第3期|p.257-263|共7页
  • 作者

    BO GAO; WEN-BIN LIU; LING-YUN JIA; LI XU; JIAN XIE;

  • 作者单位

    School of Life Science and Biotechnology, Dalian University of Technology, Dalian, P. R. China;

    School of Life Science and Biotechnology, Dalian University of Technology, Dalian, P. R. China;

    School of Life Science and Biotechnology, Dalian University of Technology, Dalian, P. R. China;

    School of Life Science and Biotechnology, Dalian University of Technology, Dalian, P. R. China;

    School of Life Science and Biotechnology, Dalian University of Technology, Dalian, P. R. China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    ddts; biodegradation; aerobic degradation; alcaligenes sp;

    机译：ddts;生物降解有氧降解;产碱菌;

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