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首页> 美国卫生研究院文献>Heliyon >Comparative analysis of antioxidant and antiproliferative activities of crude and purified flavonoid enriched fractions of pods/seeds of two desert legumes Prosopis cineraria and Cyamopsis tetragonoloba
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Comparative analysis of antioxidant and antiproliferative activities of crude and purified flavonoid enriched fractions of pods/seeds of two desert legumes Prosopis cineraria and Cyamopsis tetragonoloba

机译：两种沙漠豆科植物植物植物植物植物植物植物植物植物植物富含类富含类植物的比较分析

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摘要

Cyamopsis tetragonoloba and Prosopis cineraria are two legumes of the semi-arid region of Indian subcontinent which are unexplored with respect to their medicinal potential. Moreover, there is considerable lack in the comparative analysis of the biological properties of crude and enriched fractions obtained from the pods and seeds. Therefore, this study aims in investigating the effect of purification on the antioxidant and anticancerous activities of the extracts from the two legumes. This is the first study to purify an enriched methanolic fraction using Amberlite XAD7HP column chromatography followed by analysis using Thin Layer Chromatography. This matrix provided an economic and time efficient isolation of flavonoids and isoflavonoids from the seeds and pods of the above mentioned legumes. In addition, antioxidant activity carried out using DPPH assay showed that purification process did not contributed to enhanced antioxidant potential. However, inverse results were obtained during anticancerous activity assay on Huh-7 cell lines.
机译：Cyomopsis Tetrapoloba和Prosopis cineraria是印度次大陆半干旱地区的两个豆类，这是对其药用潜力的未开发。此外，在豆荚和种子中获得的粗额和富集级分的生物学性质的比较分析中存在相当大的缺乏。因此，本研究旨在研究纯化对两种豆类萃取物的抗氧化剂和抗遗嘱的影响。这是第一种使用琥珀酸铋Xad7HP柱色谱法纯化富集甲醇馏分的研究，然后使用薄层色谱法分析。该基质提供了来自上述豆类的种子和豆荚的黄酮类化合物和异黄酮的经济和时间有效地分离。此外，使用DPPH测定进行的抗氧化活性显示纯化过程没有导致增强的抗氧化潜力。然而，在Huh-7细胞系中抗冒犯活性测定期间获得了反应结果。

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