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首页> 美国卫生研究院文献>Plants >Transgenic Expression of dsRNA Targeting the Pentalonia nigronervosa acetylcholinesterase Gene in Banana and Plantain Reduces Aphid Populations
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Transgenic Expression of dsRNA Targeting the Pentalonia nigronervosa acetylcholinesterase Gene in Banana and Plantain Reduces Aphid Populations

机译：DsRNA的转基因表达靶向香蕉和植物中的PentalOnia nigronervosa乙酰胆碱酯酶基因减少了蚜虫种群

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摘要

The banana aphid, Pentalonia nigronervosa, is the sole insect vector of banana bunchy top virus (BBTV), the causal agent of banana bunchy top disease. The aphid acquires and transmits BBTV while feeding on infected banana plants. RNA interference (RNAi) enables the generation of pest and disease-resistant crops; however, its effectiveness relies on the identification of pivotal gene sequences to target and silence. Acetylcholinesterase (AChE) is an essential enzyme responsible for the hydrolytic metabolism of the neurotransmitter acetylcholine in animals. In this study, the AChE gene of the banana aphid was targeted for silencing by RNAi through transgenic expression of AChE dsRNA in banana and plantain plants. The efficacy of dsRNA was first assessed using an artificial feeding assay. In vitro aphid feeding on a diet containing 7.5% sucrose, and sulfate complexes of trace metals supported aphid growth and reproduction. When AChE dsRNA was included in the diet, a dose of 500 ng/μL was lethal to the aphids. Transgenic banana cv. Cavendish Williams and plantain cvs. Gonja Manjaya and Orishele expressing AChE dsRNA were regenerated and assessed for transgene integration and copy number. When aphids were maintained on elite transgenic events, there was a 67.8%, 46.7%, and 75.6% reduction in aphid populations growing on Cavendish Williams, Gonja Manjaya, and Orishele cultivars, respectively, compared to those raised on nontransgenic control plants. These results suggest that RNAi targeting an essential aphid gene could be a useful means of reducing both aphid infestation and potentially the spread of the disease they transmit.
机译：香蕉蚜虫，Pentalonia nigronervosa，是香蕉束顶病毒（BBTV）的唯一昆虫载体，香蕉束顶级疾病的因果因子。蚜虫在喂养受感染的香蕉植物时获取并传输BBTV。 RNA干扰（RNAi）能够产生害虫和抗病作物;然而，其有效性依赖于鉴定枢转基因序列以靶向和沉默。乙酰胆碱酯酶（ACHE）是负责动物中神经递质乙酰胆碱的水解代谢的必要酶。在这项研究中，香蕉蚜虫的疼痛基因旨在通过香蕉和植物植物的疼痛DsRNA的转基因表达来沉默于RNAi。首先使用人工饲养测定评估DSRNA的功效。在含有7.5％蔗糖的饮食上的体外蚜虫，以及痕量金属的硫酸盐复合物支持蚜虫生长和繁殖。当饮食中包含疼痛DSRNA时，将500ng /μl的剂量致死至蚜虫。转基因香蕉cv。 Caventish Williams和Plantain CVS。对表达Ache DsRNA的Gonja Manjaya和Orishele进行了再生和评估转基因集成和拷贝数。当蚜虫维持在Elite转基因事件上时，与在非转基因对照植物上饲养的人相比，蚜虫威廉姆斯的蚜虫种群减少了67.8％，46.7％和75.6％和75.6％，蚜虫植物和奥里什勒品种。这些结果表明，靶向必需蚜虫基因的RNAi可能是降低蚜虫侵扰的有用手段，并且可能潜在地传播的疾病的传播。

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