• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>The Journal of Biological Chemistry >Carbon Monoxide-releasing Molecule-3 (CORM-3; Ru(CO)3Cl(Glycinate)) as a Tool to Study the Concerted Effects of Carbon Monoxide and Nitric Oxide on Bacterial Flavohemoglobin Hmp
【2h】

Carbon Monoxide-releasing Molecule-3 (CORM-3; Ru(CO)3Cl(Glycinate)) as a Tool to Study the Concerted Effects of Carbon Monoxide and Nitric Oxide on Bacterial Flavohemoglobin Hmp

机译：一氧化碳释放分子3（CORM-3; Ru（CO）3Cl（甘氨酸））作为研究一氧化碳和一氧化氮对细菌黄酮血红蛋白Hmp协同作用的工具

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

CO and NO are small toxic gaseous molecules that play pivotal roles in biology as gasotransmitters. During bacterial infection, NO, produced by the host via the inducible NO synthase, exerts critical antibacterial effects while CO, generated by heme oxygenases, enhances phagocytosis of macrophages. In Escherichia coli, other bacteria and fungi, the flavohemoglobin Hmp is the most important detoxification mechanism converting NO and O2 to the ion nitrate (NO3−). The protoheme of Hmp binds not only O2 and NO, but also CO so that this ligand is expected to be an inhibitor of NO detoxification in vivo and in vitro. CORM-3 (Ru(CO)3Cl(glycinate)) is a metal carbonyl compound extensively used and recently shown to have potent antibacterial properties. In this study, attenuation of the NO resistance of E. coli by CORM-3 is demonstrated in vivo. However, polarographic measurements showed that CO gas, but not CORM-3, produced inhibition of the NO detoxification activity of Hmp in vitro. Nevertheless, CO release from CORM-3 in the presence of soluble cellular compounds is demonstrated by formation of carboxy-Hmp. We show that the inability of CORM-3 to inhibit the activity of purified Hmp is due to slow release of CO in protein solutions alone i.e. when sodium dithionite, widely used in previous studies of CO release from CORM-3, is excluded. Finally, we measure intracellular CO released from CORM-3 by following the formation of carboxy-Hmp in respiring cells. CORM-3 is a tool to explore the concerted effects of CO and NO in vivo.
机译：一氧化碳和一氧化氮是有毒的小分子气体，在生物学中作为气体传输剂发挥着关键作用。在细菌感染期间，宿主通过可诱导的NO合酶产生的NO发挥了关键的抗菌作用，而血红素加氧酶产生的CO则增强了巨噬细胞的吞噬作用。在大肠杆菌，其他细菌和真菌中，黄素血红蛋白Hmp是将NO和O2转化为硝酸根离子（NO3 -）的最重要的排毒机制。 Hmp的原血红素不仅与O2和NO结合，而且与CO结合，因此该配体有望成为体内和体外NO排毒的抑制剂。 CORM-3（Ru（CO）3Cl（甘氨酸盐））是广泛使用的羰基金属化合物，最近被证明具有强大的抗菌性能。在这项研究中，在体内证明了CORM-3对大肠杆菌NO耐药性的减弱。然而，极谱测量显示，CO气体而不是CORM-3在体外对Hmp的NO解毒活性产生抑制作用。然而，在可溶性细胞化合物存在下，CORM-3释放出的CO可以通过形成羧基Hmp来证明。我们表明，CORM-3不能抑制纯化的Hmp的活性是由于单独的蛋白质溶液中CO的缓慢释放所致，即，排除了连二亚硫酸钠（以前广泛用于CORM-3的CO释放研究中）。最后，我们通过在呼吸细胞中形成羧基-Hmp来测量从CORM-3释放的细胞内CO。 CORM-3是探索体内CO和NO协同作用的工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号