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首页> 美国卫生研究院文献>Antioxidants >UHPLC-ESI-QTOF-MS/MS Metabolite Profiling of the Antioxidant and Antidiabetic Activities of Red Cabbage and Broccoli Seeds and Sprouts
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UHPLC-ESI-QTOF-MS/MS Metabolite Profiling of the Antioxidant and Antidiabetic Activities of Red Cabbage and Broccoli Seeds and Sprouts

机译：uhplc-esi-qtof-ms / ms代谢物分析的红甘蓝和西兰花种子和豆芽的抗氧化剂和抗糖尿病活性

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The antioxidant and antidiabetic properties and metabolite profiling of ethanol extracts of red cabbage (RC) and broccoli (BR) seeds and sprouts were investigated in this study. The total phenolic, flavonoid, and saponin contents were in the ranges of 385.4–480.4 mg FAE/100 g, 206.9–215.6 mg CE/100 g, and 17.8–27.0 mg soysaponin BE/100 g, respectively. BR seed had the highest total phenolic (480.4 mg FAE/100 g) and flavonoid (216.9 mg CE/100 g) contents, whereas BR sprout had the highest saponin content (27.0 soysaponin BE/100g). RC sprout demonstrated the highest antioxidant capacity, with DPPH and ABTS radical scavenging activity levels of 71.5% and 88.5%, respectively. Furthermore, BR and RC sprouts showed the most potent inhibition against α-glucosidase (91.32% and 93.11%, respectively) and pancreatic lipase (60.19% and 61.66%, respectively). BR seed (60.37%) demonstrated the lowest AGE inhibition. A total of 24 metabolites, predominantly amino acids and phenolic compounds, were characterized using UHPLC-QTOF-MS/MS. Germination not only improved the levels of metabolites but also resulted in the synthesis of new compounds. Therefore, these findings show that germination effectively enhanced the functional properties and metabolite profiles of broccoli and red cabbage seeds, making their sprouts more applicable as functional ingredients.
机译：在本研究中研究了红甘蓝（RC）和西兰花（BR）种子和豆芽的乙醇提取物的抗氧化和抗透析性能和代谢物分析。总酚类，黄酮和皂苷含量分别为385.4-480.4 mg FAE / 100g，206.9-215.6mg Ce / 100g和17.8-27.0mg Soysaponin的范围为/ 100g。 BR种子具有最高的总酚（480.4毫克FAE / 100g）和类黄酮（216.9毫克CE / 100克）的内容，而BR发芽具有最高的皂苷含量（27.0大豆皂甙BE / 100克）。 RC Sprout分别显示出最高的抗氧化能力，DPPH和ABTS分别为71.5％和88.5％的激进清除活性水平。此外，BR和RC豆芽对α-葡糖苷酶（分别为91.32％和93.11％）和胰脂肪酶分别的最有效的抑制（分别为60.19％和61.66％）。 BR种子（60.37％）显示出最低的年龄抑制。使用UHPLC-QTOF-MS / MS为总共24种代谢物，主要是氨基酸和酚类化合物。发芽不仅改善了代谢物的水平，而且导致新化合物的合成。因此，这些发现表明，萌发有效增强了西兰花和红甘蓝种子的功能性质和代谢物谱，使得它们的萌芽更适用于功能性成分。

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