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首页> 美国卫生研究院文献>International Journal of Bioprinting >Graphene Oxide-Embedded Extracellular Matrix-Derived Hydrogel as a Multiresponsive Platform for 3D Bioprinting Applications
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Graphene Oxide-Embedded Extracellular Matrix-Derived Hydrogel as a Multiresponsive Platform for 3D Bioprinting Applications

机译：将石墨烯氧化嵌入的细胞外基质衍生的水凝胶作为3D生物印刷机应用的多型平台

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Decellularized extracellular matrices (dECMs) have shown enormous potential for the biofabrication of tissues due to their biomimetic properties that promote enhanced cellular interaction and tissue regeneration. However, biofabrication schemes requiring electrostimulation pose an additional constraint due to the insulating properties of natural materials. Here, we propose a methacryloyl-modified decellularized small intestine submucosa (SISMA) hydrogel, embedded with graphene oxide (GO) nanosheets, for extrusion-based 3D bioprinting applications that require electrostimulation. Methacryloyl biochemical modification is performed to enhance the mechanical stability of dECM constructs by mediating photo-crosslinking reactions, and a multistep fabrication scheme is proposed to harness the bioactive and hydrophilic properties of GO and electroconductive properties of reduced GO. For this, GO was initially dispersed in SISMA hydrogels by exploiting its hydrophilicity and protein adsorption capabilities, and in situ reduction was subsequently performed to confer electroconductive abilities. SISMA-GO composite hydrogels were successfully prepared with enhanced structural characteristics, as shown by the higher crosslinking degree and increased elastic response upon blue-light exposure. Moreover, GO was homogeneously dispersed without affecting photocrosslinking reactions and hydrogel shear-thinning properties. Human adipose-derived mesenchymal stem cells were successfully bioprinted in SISMA-GO with high cell viability after 1 week and in situ reduction of GO during this period enhanced the electrical conductivity of these nanostructures. This work demonstrates the potential of SISMA-GO bioinks as bioactive and electroconductive scaffolds for electrostimulation applications in tissue engineering and regenerative medicine.
机译：由于它们的培养性质的性质，脱细胞化细胞外基质（Decms）表明了组织的生物结缔组织的巨大潜力。促进细胞相互作用和组织再生的仿生性能。然而，由于天然材料的绝缘性能，需要电刺激的生物制作方案占额外的约束。在此，我们提出了一种甲基丙烯酰基改性的小肠粘膜下皮（SISMA）水凝胶，嵌入着石墨烯氧化物（GO）纳米片，用于需要电动刺激的基于挤出的3D生物监测应用。进行甲基丙烯酰基生物化学修饰以增强DECM构建体的机械稳定性，通过介导光交联反应，提出了多步制制造方案来利用Go的生物活性和亲水性能和减少的导电性能。为此，GO最初通过利用其亲水性和蛋白质吸附能力分散在SISMA水凝胶中，随后进行了原位还原以赋予导电能力。通过增强的结构特性成功制备SISMA-GO复合水凝胶，如较高的交联度和增加蓝光暴露时的弹性响应所示。此外，GO均匀地分散而不影响光关键反应和水凝胶剪切稀疏性能。人脂肪衍生的间充质干细胞在SISMA-GO中成功地生成了在1周后的高细胞活力，并且在该时段期间，在该时段的情况下，增强了这些纳米结构的电导率。这项工作证明了SISMA-GO生物链的潜力作为用于组织工程和再生医学中的用于静电和导电支架的生物活性和导电支架。

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