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首页> 美国卫生研究院文献>Journal of Bacteriology >Inhibition of Formation of Escherichia coli Mating Pairs by f1 and MS2 Bacteriophages as Determined with a Coulter Counter
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Inhibition of Formation of Escherichia coli Mating Pairs by f1 and MS2 Bacteriophages as Determined with a Coulter Counter

机译：通过库尔特计数器确定的f1和MS2噬菌体对大肠杆菌交配对形成的抑制作用

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The effect of male-specific filamentous deoxyribonucleic acid (f1) and isometric ribonucleic acid (MS2) bacteriophages on the formation of mating pairs in Escherichia coli conjugation was examined directly in the Coulter counter. When a sufficient multiplicity of infection (MOI) was used, the f1 phage immediately and completely inhibited the formation of mating pairs. On the other hand, the MS2 phage at a relatively high MOI also inhibited the formation of mating pairs significantly although not completey. The inhibitory effect of MS2 phage was dependent on the time of addition and the MOI used. At relatively low MOI (<20), the MS2 phage showed some inhibitory effect when added to a male culture prior to mixing with females, whereas no effect was observed when phages were added after mating pair formation had already commenced. At a high MOI (>400) MS2 phage disrupted the mating pairs already formed. Some preformed mating pairs were resistant to the high MOI of MS2 phages, however, and the “sensitive” (to high MOI) mating pairs seem to mature into “resistant” mating pairs as a function of time. We conclude that the tip of an F pilus is the specific attachment site for mating. The following process of mating pair formation has been formulated by deduction. (i) The sides of F pili weakly contact female cells, (ii) then the tips of F pili attach to the specific receptor sites to form initial mating pairs, and (iii) those pairs mature into mating pairs that are resistant to the high MOI of MS2 phages. The high MOI of MS2 prevents the first step, whereas f1 phages affect the second step—the binding between the tips of F pili and the receptor sites.
机译：直接在库尔特计数器中检查了雄性特异性丝状脱氧核糖核酸（f1）和等距核糖核酸（MS2）噬菌体对大肠杆菌结合中交配对形成的影响。当使用足够多的感染（MOI）时，f1噬菌体立即并完全抑制了配对的形成。另一方面，相对较高的MOI的MS2噬菌体也显着抑制了配对对的形成，尽管还不完全。 MS2噬菌体的抑制作用取决于添加时间和所用的MOI。在相对较低的MOI（<20）下，将MS2噬菌体添加到雄性培养物中与雌性混合之前，表现出一定的抑制作用，而当已经开始形成配对时，添加噬菌体则没有观察到抑制作用。 MOI（> 400）较高时，MS2噬菌体破坏了已经形成的配对。一些预先形成的配对对MS2噬菌体的高MOI具有抗性，但是，随着时间的推移，“敏感”（对高MOI）配对对似乎已成熟为“抗性”配对。我们得出的结论是F菌毛的尖端是交配的特定附着部位。通过推导已制定了以下配对过程。 （i）F菌毛的侧面与雌性细胞较弱地接触，（ii）然后F菌毛的末端附着于特定的受体位点以形成初始的交配对，并且（iii）这些对成熟成对高抗性的交配对MS2噬菌体的MOI。 MS2的高MOI阻止了第一步，而f1噬菌体影响了第二步-F菌毛末端与受体位点之间的结合。

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