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首页> 美国卫生研究院文献>Journal of Bacteriology >Methylase activities from Haemophilus influenzae that protect Haemophilus parainfluenzae transforming deoxyribonucleic acid from inactivation by Haemophilus influenzae endonuclease R.
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Methylase activities from Haemophilus influenzae that protect Haemophilus parainfluenzae transforming deoxyribonucleic acid from inactivation by Haemophilus influenzae endonuclease R.

机译：流感嗜血杆菌的甲基化酶活性可保护副流感嗜血杆菌转化脱氧核糖核酸免受流感嗜血杆菌核酸内切酶R的灭活。

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摘要

Specific methylases that have the properties of deoxyribonucleic acid (DNA) modification enzymes have been isolated from Haemophilus influenzae strain Rd. Two activities ((Methylase IIa and methylase III) were found to protect transforming DNA of H. parainfluenzae from the action of H. influenzae restriction enzymes. To determine the specificty of the protection, a procedure based on biological activity was developed for the separation and purification of the restriction endonucleases from H. influenzae strain Rd. Two endonuclease R activities presumably corresponding to Hind II and Hind III (P. H. Roy and H. O. Smith, 1973; H. O. Smith and K. W. Wilcox, 1970) were characterized by differences in their chromatographic properties, ability to attack T7 DNA, and inactivation of the transforming activity of different markers of H. parainfluenzae DNA. One endonuclease R enzyme (Hind II) attacked T7 DNA and was found to inactivate the dalacin resistance marker (smaller than 0.01% activity remaining) with only a slight effect on the streptomycin resistance marker (83% activity remaining). Methylase IIa treatment protected 40% of the dalacin resistance marker of H. parainfluenzae DNA from inactivation by Hind II. The other restriction activity (Hind III) was inert towards T7 DNA and inactivated the streptomycin resistance marker of H. parainfluenzae DNA (smaller than 0.01% activity remaining) without any effect on the dalacin resistance marker. The methylation of H. parainfluenzae DNA accomplished by methylase III protected 60% of the transforming activity of the streptomycin resistance marker of H. parainfluenzae DNA from the action of Hind III.
机译：具有脱氧核糖核酸（DNA）修饰酶特性的特定甲基化酶已从流感嗜血杆菌菌株Rd分离。发现了两种活性（（甲基化酶IIa和甲基化酶III）可以保护副流感嗜血杆菌的转化DNA免受流感嗜血杆菌限制性内切酶的作用，为确定保护的特异性，开发了一种基于生物学活性的分离和分离方法。从流感嗜血杆菌菌株Rd纯化限制性核酸内切酶。据推测，两种核酸内切酶R活性分别对应于Hind II和Hind III（PH Roy和HO Smith，1973； HO Smith和KW Wilcox，1970），其色谱特性不同，攻击T7 DNA的能力，以及副流感嗜血杆菌DNA不同标记的转化活性失活一种核酸内切酶R酶（Hind II）攻击T7 DNA，发现其使达拉新抗性标记失活（剩余活性小于0.01％），仅对链霉素抗性标记有轻微影响（剩余83％的活性）甲基化酶IIa处理可保护40％的达拉金水库Hind II灭活的副流感嗜血杆菌DNA的等距标记。其他限制酶活性（Hind III）对T7 DNA呈惰性，并灭活了副流感嗜血杆菌DNA的链霉素抗性标记（剩余活性低于0.01％），而对达拉胶抗性标记没有任何影响。通过甲基化酶III完成的副流感嗜血菌DNA的甲基化保护了副流感嗜血菌DNA的链霉素抗性标记的60％的转化活性免于Hind III的作用。

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