Troubleshooting LTQ and LTQ-Orbitrap LC-MS/MS Systems with MassQC

机译：使用MassQC对LTQ和LTQ-Orbitrap LC-MS / MS系统进行故障排除

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>RP-54Troubleshooting liquid chromatography tandem mass spectrometry (LC-MS/MS) systems is a top priority in academic and commercial proteomics laboratories. We present a new software tool, MassQC, that builds on work from the National Institute of Standards and Technology (NIST) to provide an easy-to-use and powerful method to evaluate and track LC-MS/MS system performance over time. Using this novel approach we explore three common system failure modes (including spray instability, detector sensitivity degradation and erratic peptide elution) that were detected by MassQC while operating Thermo LTQ and LTQ-Orbitrap LC-MS/MS instruments. While viewing total ion current or base peak intensity may or may not shed light on these problems, MassQC alerts you to out-of-range metrics through robust trend tracking. Further, MassQC proved valuable for troubleshooting common system performance problems and optimizing LC-MS/MS instrument operation for improving proteomics reproducibility.

机译：> RP-54 对液相色谱串联质谱（LC-MS / MS）系统进行故障排除是学术和商业蛋白质组学实验室的重中之重。我们提出了一种新的软件工具MassQC，该工具以美国国家标准技术研究院（NIST）的工作为基础，旨在提供一种易于使用且功能强大的方法来评估和跟踪LC-MS / MS系统的性能。使用这种新颖的方法，我们探索了在操作Thermo LTQ和LTQ-Orbitrap LC-MS / MS仪器时MassQC检测到的三种常见系统故障模式（包括喷雾不稳定性，检测器灵敏度下降和不稳定的肽洗脱）。在查看总离子流或基峰强度可能会或可能不会发现这些问题时，MassQC会通过可靠的趋势跟踪提醒您超出范围的指标。此外，MassQC被证明对于解决常见的系统性能问题和优化LC-MS / MS仪器操作以提高蛋白质组学的可重复性具有重要意义。

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期刊名称 Journal of Biomolecular Techniques : JBT
作者
J. Klimek; C.J. Mason; N. Vincent-Maloney;
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年(卷),期 2010(21),3 Suppl
年度 2010
页码 S37
总页数 1
原文格式 PDF
正文语种
中图分类生物学;
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专利

1. Increasing the mass accuracy of high-resolution LC-MS data using background ions - A case study on the LTQ-Orbitrap [J] . ScheltemaR.A., KamlehA., WildridgeD., Proteomics . 2008,第22期

机译：使用背景离子提高高分辨率LC-MS数据的质量准确性-以LTQ-Orbitrap为例
2. Improving protein identification sensitivity by combining MS and MS/MS information for shotgun proteomics using LTQ-Orbitrap high mass accuracy data [J] . Lu BW, Motoyama A, Ruse C, Analytical chemistry . 2008,第6期

机译：通过结合使用LTQ-Orbitrap高质量数据的shot弹枪蛋白质组学的MS和MS / MS信息来提高蛋白质识别灵敏度
3. Multi-component identification and target cell-based screening of potential bioactive compounds in toad venom by UPLC coupled with high-resolution LTQ-Orbitrap MS and high-sensitivity Qtrap MS [J] . Ren Wei, Han Lingyu, Luo Mengyi, Analytical and bioanalytical chemistry . 2018,第18期

机译：通过UPLC与高分辨率LTQ-orbitrap MS和高灵敏度QTRAP MS的UPLC耦合蟾蜍毒液中潜在生物活性化合物的多组分鉴定和基于靶细胞的筛选
4. Investigation of the Metabolism of TZP-101 in Healthy Volunteers after Multiple Intravenous Doses by Applying Complementary LC-MS/MS on an API 4000 Qtrap and an LTQ-Orbitrap [C] . Ming Qi, Mark Gohdes, Ryan Berko, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2010

机译：通过在API 4000 QTRAP施用互补LC-MS / MS和LTQ-orbitrap施用互补LC-MS / MS后，在多静脉内剂量后对健康志愿者进行TZP-101的代谢调查
5. LC-MS/MS/MS Quantification of Oxidatively Induced DNA Lesions [D] . Guerrero, Candace Rae 2014

机译：LC-MS / MS / MS定量测定氧化性DNA损伤
6. Improving Protein Identification Sensitivity by Combining MS and MS/MS Information for Shotgun Proteomics Using LTQ-Orbitrap High Mass Accuracy Data [O] . Bingwen Lu, Akira Motoyama, Cristian Ruse, -1

机译：通过结合使用LTQ-Orbitrap高质量数据的Shot弹枪蛋白质组学的MS和MS / MS信息来提高蛋白质鉴定灵敏度
7. Increasing the mass accuracy of high-resolution LC-MS data using background ions - a case study on the LTQ-Orbitrap [O] . Scheltema Richard A., Kamleh Anas, Wildridge David, 2008

机译：使用背景离子提高高分辨率LC-MS数据的质量准确性-以LTQ-Orbitrap为例

Troubleshooting LTQ and LTQ-Orbitrap LC-MS/MS Systems with MassQC

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